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Molecular & cellular proteomics : MCP DOI:10.1074/mcp.R111.009522

A face in the crowd: recognizing peptides through database search.

Publication TypeJournal Article
Year of Publication2011
AuthorsEng, JK, Searle, BC, Clauser, KR, Tabb, DL
JournalMolecular & cellular proteomics : MCP
Volume10
Issue11
PagesR111.009522
Date Published2011/11/01
ISSN1535-9476
Abstract

Peptide identification via tandem mass spectrometry sequence database searching is a key method in the array of tools available to the proteomics researcher. The ability to rapidly and sensitively acquire tandem mass spectrometry data and perform peptide and protein identifications has become a commonly used proteomics analysis technique because of advances in both instrumentation and software. Although many different tandem mass spectrometry database search tools are currently available from both academic and commercial sources, these algorithms share similar core elements while maintaining distinctive features. This review revisits the mechanism of sequence database searching and discusses how various parameter settings impact the underlying search.

URLhttp://www.mcponline.org/cgi/pmidlookup?view=long&pmid=21876205
DOI10.1074/mcp.R111.009522
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/21876205?dopt=Abstract