|Publication Type||Journal Article|
|Year of Publication||2014|
|Authors||Hoeppner, MP, Lundquist, A, Pirun, M, Meadows, JRS, Zamani, N, Johnson, J, Sundström, G, Cook, A, FitzGerald, MG, Swofford, R, Mauceli, E, Moghadam, BTorabi, Greka, A, Alföldi, J, Abouelleil, A, Aftuck, L, Bessette, D, Berlin, A, Brown, A, Gearin, G, Lui, A, J Macdonald, P, Priest, M, Shea, T, Turner-Maier, J, Zimmer, A, Lander, ES, di Palma, F, Lindblad-Toh, K, Grabherr, MG|
|Keywords||Animals, Cell Line, Dogs, Exons, Gene Expression Profiling, Genome, Humans, Mice, Nerve Tissue Proteins, Oligonucleotides, Antisense, Podocytes, Polymorphism, Single Nucleotide, RNA, Messenger, RNA, Small Interfering, RNA, Untranslated, Sequence Analysis, RNA|
The domestic dog, Canis familiaris, is a well-established model system for mapping trait and disease loci. While the original draft sequence was of good quality, gaps were abundant particularly in promoter regions of the genome, negatively impacting the annotation and study of candidate genes. Here, we present an improved genome build, canFam3.1, which includes 85 MB of novel sequence and now covers 99.8% of the euchromatic portion of the genome. We also present multiple RNA-Sequencing data sets from 10 different canine tissues to catalog ∼175,000 expressed loci. While about 90% of the coding genes previously annotated by EnsEMBL have measurable expression in at least one sample, the number of transcript isoforms detected by our data expands the EnsEMBL annotations by a factor of four. Syntenic comparison with the human genome revealed an additional ∼3,000 loci that are characterized as protein coding in human and were also expressed in the dog, suggesting that those were previously not annotated in the EnsEMBL canine gene set. In addition to ∼20,700 high-confidence protein coding loci, we found ∼4,600 antisense transcripts overlapping exons of protein coding genes, ∼7,200 intergenic multi-exon transcripts without coding potential, likely candidates for long intergenic non-coding RNAs (lincRNAs) and ∼11,000 transcripts were reported by two different library construction methods but did not fit any of the above categories. Of the lincRNAs, about 6,000 have no annotated orthologs in human or mouse. Functional analysis of two novel transcripts with shRNA in a mouse kidney cell line altered cell morphology and motility. All in all, we provide a much-improved annotation of the canine genome and suggest regulatory functions for several of the novel non-coding transcripts.
|Alternate Journal||PLoS ONE|
|PubMed Central ID||PMC3953330|
|Grant List||U54 HG003067 / HG / NHGRI NIH HHS / United States|