RNA-guided genome editing of mammalian cells.

Methods Mol Biol

Authors	Neena Pyzocha F Ann Ran Patrick Hsu Feng Zhang
Keywords	Animals Humans Cell Line Genome Genetic Vectors RNA, Guide Cloning, Molecular Transfection Gene Targeting RNA Editing Gene Order DNA End-Joining Repair
Abstract	The microbial CRISPR-Cas adaptive immune system can be harnessed to facilitate genome editing in eukaryotic cells (Cong L et al., Science 339, 819-823, 2013; Mali P et al., Science 339, 823-826, 2013). Here we describe a protocol for the use of the RNA-guided Cas9 nuclease from the Streptococcus pyogenes type II CRISPR system to achieve specific, scalable, and cost-efficient genome editing in mammalian cells.
Year of Publication	2014
Journal	Methods Mol Biol
Volume	1114
Pages	269-77
Date Published	2014
ISSN	1940-6029
URL	http://dx.doi.org/10.1007/978-1-62703-761-7_17
DOI	10.1007/978-1-62703-761-7_17
PubMed ID	24557909
Links	PubMed Google Scholar DOI
Grant list	DP1-MH100706 / DP / NCCDPHP CDC HHS / United States R01-NS073124 / NS / NINDS NIH HHS / United States

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