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Methods in molecular biology (Clifton, N.J.) DOI:10.1007/978-1-62703-761-7_17

RNA-Guided Genome Editing of Mammalian Cells.

Publication TypeJournal Article
Year of Publication2014
AuthorsPyzocha, NK, Ran, FA, Hsu, PD, Zhang, F
JournalMethods in molecular biology (Clifton, N.J.)
Volume1114
Pages269-77
Date Published2014/01/01
ISSN1064-3745
Abstract

The microbial CRISPR-Cas adaptive immune system can be harnessed to facilitate genome editing in eukaryotic cells (Cong L et al., Science 339, 819-823, 2013; Mali P et al., Science 339, 823-826, 2013). Here we describe a protocol for the use of the RNA-guided Cas9 nuclease from the Streptococcus pyogenes type II CRISPR system to achieve specific, scalable, and cost-efficient genome editing in mammalian cells.

URLhttp://dx.doi.org/10.1007/978-1-62703-761-7_17
DOI10.1007/978-1-62703-761-7_17
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/24557909?dopt=Abstract