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Methods Mol Biol DOI:10.1007/978-1-62703-761-7_17

RNA-guided genome editing of mammalian cells.

Publication TypeJournal Article
Year of Publication2014
AuthorsPyzocha, NK, F Ran, A, Hsu, PD, Zhang, F
JournalMethods Mol Biol
Date Published2014
KeywordsAnimals, Cell Line, Cloning, Molecular, DNA End-Joining Repair, Gene Order, Gene Targeting, Genetic Vectors, Genome, Humans, RNA Editing, RNA, Guide, Transfection

The microbial CRISPR-Cas adaptive immune system can be harnessed to facilitate genome editing in eukaryotic cells (Cong L et al., Science 339, 819-823, 2013; Mali P et al., Science 339, 823-826, 2013). Here we describe a protocol for the use of the RNA-guided Cas9 nuclease from the Streptococcus pyogenes type II CRISPR system to achieve specific, scalable, and cost-efficient genome editing in mammalian cells.


Alternate JournalMethods Mol. Biol.
PubMed ID24557909
Grant ListDP1-MH100706 / DP / NCCDPHP CDC HHS / United States
R01-NS073124 / NS / NINDS NIH HHS / United States