|Publication Type||Journal Article|
|Year of Publication||2014|
|Authors||Hacisuleyman, E, Goff, LA, Trapnell, C, Williams, A, Henao-Mejia, J, Sun, L, McClanahan, P, Hendrickson, DG, Sauvageau, M, Kelley, DR, Morse, M, Engreitz, J, Lander, ES, Guttman, M, Lodish, HF, Flavell, R, Raj, A, Rinn, JL|
|Journal||Nature structural & molecular biology|
RNA, including long noncoding RNA (lncRNA), is known to be an abundant and important structural component of the nuclear matrix. However, the molecular identities, functional roles and localization dynamics of lncRNAs that influence nuclear architecture remain poorly understood. Here, we describe one lncRNA, Firre, that interacts with the nuclear-matrix factor hnRNPU through a 156-bp repeating sequence and localizes across an ~5-Mb domain on the X chromosome. We further observed Firre localization across five distinct trans-chromosomal loci, which reside in spatial proximity to the Firre genomic locus on the X chromosome. Both genetic deletion of the Firre locus and knockdown of hnRNPU resulted in loss of colocalization of these trans-chromosomal interacting loci. Thus, our data suggest a model in which lncRNAs such as Firre can interface with and modulate nuclear architecture across chromosomes.