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Nature DOI:10.1038/nature12881

Landscape of genomic alterations in cervical carcinomas.

Publication TypeJournal Article
Year of Publication2014
AuthorsOjesina, AI, Lichtenstein, L, Freeman, SS, Pedamallu, CSekhar, Imaz-Rosshandler, I, Pugh, TJ, Cherniack, AD, Ambrogio, L, Cibulskis, K, Bertelsen, B, Romero-Cordoba, S, Treviño, V, Vazquez-Santillan, K, Guadarrama, ASalido, Wright, AA, Rosenberg, MW, Duke, F, Kaplan, B, Wang, R, Nickerson, E, Walline, HM, Lawrence, MS, Stewart, C, Carter, SL, McKenna, A, Rodriguez-Sanchez, IP, Espinosa-Castilla, M, Woie, K, Bjorge, L, Wik, E, Halle, MK, Hoivik, EA, Krakstad, C, Gabiño, NBelem, Gómez-Macías, GSofia, Valdez-Chapa, LD, Garza-Rodríguez, MLourdes, Maytorena, G, Vazquez, J, Rodea, C, Cravioto, A, Cortés, ML, Greulich, H, Crum, CP, Neuberg, DS, Hidalgo-Miranda, A, Escareno, CRangel, Akslen, LA, Carey, TE, Vintermyr, OK, Gabriel, SB, Barrera-Saldaña, HA, Melendez-Zajgla, J, Getz, G, Salvesen, HB, Meyerson, M
Date Published2014 Feb 20
KeywordsAdenocarcinoma, Carcinoma, Squamous Cell, Case-Control Studies, Cell Cycle Proteins, Core Binding Factor beta Subunit, DNA Copy Number Variations, DNA Mutational Analysis, DNA-Binding Proteins, E1A-Associated p300 Protein, Exome, F-Box Proteins, Female, Gene Expression Regulation, Neoplastic, Genome, Human, Genomics, HLA-B Antigens, Humans, Mitogen-Activated Protein Kinase 1, Mutation, NF-E2-Related Factor 2, Papillomaviridae, Papillomavirus Infections, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-ets, Receptor, ErbB-2, Transcription Factors, Transcriptome, Tumor Suppressor Protein p53, Ubiquitin-Protein Ligases, Uterine Cervical Neoplasms, Virus Integration

Cervical cancer is responsible for 10-15% of cancer-related deaths in women worldwide. The aetiological role of infection with high-risk human papilloma viruses (HPVs) in cervical carcinomas is well established. Previous studies have also implicated somatic mutations in PIK3CA, PTEN, TP53, STK11 and KRAS as well as several copy-number alterations in the pathogenesis of cervical carcinomas. Here we report whole-exome sequencing analysis of 115 cervical carcinoma-normal paired samples, transcriptome sequencing of 79 cases and whole-genome sequencing of 14 tumour-normal pairs. Previously unknown somatic mutations in 79 primary squamous cell carcinomas include recurrent E322K substitutions in the MAPK1 gene (8%), inactivating mutations in the HLA-B gene (9%), and mutations in EP300 (16%), FBXW7 (15%), NFE2L2 (4%), TP53 (5%) and ERBB2 (6%). We also observe somatic ELF3 (13%) and CBFB (8%) mutations in 24 adenocarcinomas. Squamous cell carcinomas have higher frequencies of somatic nucleotide substitutions occurring at cytosines preceded by thymines (Tp*C sites) than adenocarcinomas. Gene expression levels at HPV integration sites were statistically significantly higher in tumours with HPV integration compared with expression of the same genes in tumours without viral integration at the same site. These data demonstrate several recurrent genomic alterations in cervical carcinomas that suggest new strategies to combat this disease.


Alternate JournalNature
PubMed ID24390348
PubMed Central IDPMC4161954
Grant ListK07 CA166210 / CA / NCI NIH HHS / United States
T32 CA009676 / CA / NCI NIH HHS / United States