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Prostate cancer and prostatic diseases DOI:10.1038/pcan.2013.37

Successful whole-exome sequencing from a prostate cancer bone metastasis biopsy.

Publication TypeJournal Article
Year of Publication2013
AuthorsVan Allen, EM, Foye, A, Wagle, N, Kim, W, Carter, SL, McKenna, A, Simko, JP, Garraway, LA, Febbo, PG
JournalProstate cancer and prostatic diseases
Date Published2013/12/24

Background:Comprehensive molecular characterization of cancer that has metastasized to bone has proved challenging, which may limit the diagnostic and potential therapeutic opportunities for patients with bone-only metastatic disease.Methods:We describe successful tissue acquisition, DNA extraction, and whole-exome sequencing from a bone metastasis of a patient with metastatic, castration-resistant prostate cancer (PCa).Results:The resulting high-quality tumor sequencing identified plausibly actionable somatic genomic alterations that dysregulate the phosphoinostide 3-kinase pathway, as well as a theoretically actionable germline variant in the BRCA2 gene.Conclusions:We demonstrate the feasibility of diagnostic bone metastases profiling and analysis that will be required for the widespread application of prospective 'precision medicine' to men with advanced PCa.Prostate Cancer and Prostatic Disease advance online publication, 24 December 2013; doi:10.1038/pcan.2013.37.