Successful whole-exome sequencing from a prostate cancer bone metastasis biopsy.

Prostate Cancer Prostatic Dis
Authors
Keywords
Abstract

BACKGROUND: Comprehensive molecular characterization of cancer that has metastasized to bone has proved challenging, which may limit the diagnostic and potential therapeutic opportunities for patients with bone-only metastatic disease.

METHODS: We describe successful tissue acquisition, DNA extraction, and whole-exome sequencing from a bone metastasis of a patient with metastatic, castration-resistant prostate cancer (PCa).

RESULTS: The resulting high-quality tumor sequencing identified plausibly actionable somatic genomic alterations that dysregulate the phosphoinostide 3-kinase pathway, as well as a theoretically actionable germline variant in the BRCA2 gene.

CONCLUSIONS: We demonstrate the feasibility of diagnostic bone metastases profiling and analysis that will be required for the widespread application of prospective 'precision medicine' to men with advanced PCa.

Year of Publication
2014
Journal
Prostate Cancer Prostatic Dis
Volume
17
Issue
1
Pages
23-7
Date Published
2014 Mar
ISSN
1476-5608
URL
DOI
10.1038/pcan.2013.37
PubMed ID
24366412
PubMed Central ID
PMC4364998
Links
Grant list
T32 CA009172 / CA / NCI NIH HHS / United States
U01 CA157703 / CA / NCI NIH HHS / United States
5 U01 CA157703 / CA / NCI NIH HHS / United States