Successful whole-exome sequencing from a prostate cancer bone metastasis biopsy.
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Abstract | BACKGROUND: Comprehensive molecular characterization of cancer that has metastasized to bone has proved challenging, which may limit the diagnostic and potential therapeutic opportunities for patients with bone-only metastatic disease. METHODS: We describe successful tissue acquisition, DNA extraction, and whole-exome sequencing from a bone metastasis of a patient with metastatic, castration-resistant prostate cancer (PCa). RESULTS: The resulting high-quality tumor sequencing identified plausibly actionable somatic genomic alterations that dysregulate the phosphoinostide 3-kinase pathway, as well as a theoretically actionable germline variant in the BRCA2 gene. CONCLUSIONS: We demonstrate the feasibility of diagnostic bone metastases profiling and analysis that will be required for the widespread application of prospective 'precision medicine' to men with advanced PCa. |
Year of Publication | 2014
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Journal | Prostate Cancer Prostatic Dis
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Volume | 17
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Issue | 1
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Pages | 23-7
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Date Published | 2014 Mar
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ISSN | 1476-5608
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URL | |
DOI | 10.1038/pcan.2013.37
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PubMed ID | 24366412
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PubMed Central ID | PMC4364998
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Grant list | T32 CA009172 / CA / NCI NIH HHS / United States
U01 CA157703 / CA / NCI NIH HHS / United States
5 U01 CA157703 / CA / NCI NIH HHS / United States
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