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PLoS One DOI:10.1371/journal.pone.0080999

Multiplex cytological profiling assay to measure diverse cellular states.

Publication TypeJournal Article
Year of Publication2013
AuthorsGustafsdottir, SM, Ljosa, V, Sokolnicki, KL, Anthony Wilson, J, Walpita, D, Kemp, MM, Seiler, KPetri, Carrel, HA, Golub, TR, Schreiber, SL, Clemons, PA, Carpenter, AE, Shamji, AF
JournalPLoS One
Date Published2013
KeywordsCell Line, Tumor, Fluorescent Dyes, Humans, Image Processing, Computer-Assisted

Computational methods for image-based profiling are under active development, but their success hinges on assays that can capture a wide range of phenotypes. We have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery.


Alternate JournalPLoS ONE
PubMed ID24312513
PubMed Central IDPMC3847047
Grant ListRL1HG004671 / HG / NHGRI NIH HHS / United States
U54 HG005032 / HG / NHGRI NIH HHS / United States