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Nucleic Acids Res DOI:10.1093/nar/gkt1029

The Aspergillus Genome Database: multispecies curation and incorporation of RNA-Seq data to improve structural gene annotations.

Publication TypeJournal Article
Year of Publication2014
AuthorsCerqueira, GC, Arnaud, MB, Inglis, DO, Skrzypek, MS, Binkley, G, Simison, M, Miyasato, SR, Binkley, J, Orvis, J, Shah, P, Wymore, F, Sherlock, G, Wortman, JR
JournalNucleic Acids Res
Volume42
IssueDatabase issue
PagesD705-10
Date Published2014 Jan
ISSN1362-4962
KeywordsAspergillus, Databases, Genetic, Gene Expression Profiling, Genes, Fungal, Genome, Fungal, Internet, Molecular Sequence Annotation, Sequence Analysis, RNA
Abstract

The Aspergillus Genome Database (AspGD; http://www.aspgd.org) is a freely available web-based resource that was designed for Aspergillus researchers and is also a valuable source of information for the entire fungal research community. In addition to being a repository and central point of access to genome, transcriptome and polymorphism data, AspGD hosts a comprehensive comparative genomics toolbox that facilitates the exploration of precomputed orthologs among the 20 currently available Aspergillus genomes. AspGD curators perform gene product annotation based on review of the literature for four key Aspergillus species: Aspergillus nidulans, Aspergillus oryzae, Aspergillus fumigatus and Aspergillus niger. We have iteratively improved the structural annotation of Aspergillus genomes through the analysis of publicly available transcription data, mostly expressed sequenced tags, as described in a previous NAR Database article (Arnaud et al. 2012). In this update, we report substantive structural annotation improvements for A. nidulans, A. oryzae and A. fumigatus genomes based on recently available RNA-Seq data. Over 26 000 loci were updated across these species; although those primarily comprise the addition and extension of untranslated regions (UTRs), the new analysis also enabled over 1000 modifications affecting the coding sequence of genes in each target genome.

URLhttp://nar.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=24194595
DOI10.1093/nar/gkt1029
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/24194595?dopt=Abstract

Alternate JournalNucleic Acids Res.
PubMed ID24194595
PubMed Central IDPMC3965050
Grant ListR01 AI077599 / AI / NIAID NIH HHS / United States