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Nature communications DOI:10.1038/ncomms3672

Semiconductor-based DNA sequencing of histone modification states.

Publication TypeJournal Article
Year of Publication2013
AuthorsCheng, CS, Rai, K, Garber, M, Hollinger, A, Robbins, D, Anderson, S, Macbeth, A, Tzou, A, Carneiro, MO, Raychowdhury, R, Russ, C, Hacohen, N, Gershenwald, JE, Lennon, N, Nusbaum, C, Chin, L, Regev, A, Amit, I
JournalNature communications
Date Published2013/10/25

The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.