Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Cheng, CS, Rai, K, Garber, M, Hollinger, A, Robbins, D, Anderson, S, Macbeth, A, Tzou, A, Carneiro, MO, Raychowdhury, R, Russ, C, Hacohen, N, Gershenwald, JE, Lennon, N, Nusbaum, C, Chin, L, Regev, A, Amit, I |
Journal | Nat Commun |
Volume | 4 |
Pages | 2672 |
Date Published | 2013 |
ISSN | 2041-1723 |
Keywords | Animals, Chromatin, Chromatin Immunoprecipitation, Dendritic Cells, Epigenesis, Genetic, Female, Genome, Human, Histones, Humans, Melanoma, Mice, Mice, Inbred C57BL, Protein Processing, Post-Translational, Semiconductors, Sequence Analysis, DNA, Skin Neoplasms |
Abstract | The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues. |
URL | http://dx.doi.org/10.1038/ncomms3672 |
DOI | 10.1038/ncomms3672 |
Pubmed | |
Alternate Journal | Nat Commun |
PubMed ID | 24157732 |
PubMed Central ID | PMC3917140 |
Grant List | U54 HG003067 / HG / NHGRI NIH HHS / United States P30 DK043351 / DK / NIDDK NIH HHS / United States U01 CA1411508 / CA / NCI NIH HHS / United States F32 CA168253 / CA / NCI NIH HHS / United States U54HG003067 / HG / NHGRI NIH HHS / United States DP1OD003958-01 / OD / NIH HHS / United States R01 CA093947 / CA / NCI NIH HHS / United States / / Howard Hughes Medical Institute / United States P50 HG006193 / HG / NHGRI NIH HHS / United States 1P50HG006193-01 / HG / NHGRI NIH HHS / United States P50 CA093459 / CA / NCI NIH HHS / United States U01 CA141508 / CA / NCI NIH HHS / United States R01 CA93947 / CA / NCI NIH HHS / United States DP1 OD003958 / OD / NIH HHS / United States |
Nat Commun DOI:10.1038/ncomms3672
Semiconductor-based DNA sequencing of histone modification states.
Recent Broad Publications
News at the broad
News / 04.5.18
News / 04.2.18