|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Cheng, CS, Rai, K, Garber, M, Hollinger, A, Robbins, D, Anderson, S, Macbeth, A, Tzou, A, Carneiro, MO, Raychowdhury, R, Russ, C, Hacohen, N, Gershenwald, JE, Lennon, N, Nusbaum, C, Chin, L, Regev, A, Amit, I|
The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.