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PLoS Pathog DOI:10.1371/journal.ppat.1007528

Antagonistic paralogs control a switch between growth and pathogen resistance in C. elegans.

Publication TypeJournal Article
Year of Publication2019
AuthorsReddy, KC, Dror, T, Underwood, RS, Osman, GA, Elder, CR, Desjardins, CA, Cuomo, CA, Barkoulas, M, Troemel, ER
JournalPLoS Pathog
Volume15
Issue1
Pagese1007528
Date Published2019 01
ISSN1553-7374
KeywordsAnimals, Biological Evolution, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Gene Expression Profiling, Genetic Testing, Host-Pathogen Interactions
Abstract

Immune genes are under intense, pathogen-induced pressure, which causes these genes to diversify over evolutionary time and become species-specific. Through a forward genetic screen we recently described a C. elegans-specific gene called pals-22 to be a repressor of "Intracellular Pathogen Response" or IPR genes. Here we describe pals-25, which, like pals-22, is a species-specific gene of unknown biochemical function. We identified pals-25 in a screen for suppression of pals-22 mutant phenotypes and found that mutations in pals-25 suppress all known phenotypes caused by mutations in pals-22. These phenotypes include increased IPR gene expression, thermotolerance, and immunity against natural pathogens, including Nematocida parisii microsporidia and the Orsay virus. Mutations in pals-25 also reverse the reduced lifespan and slowed growth of pals-22 mutants. Transcriptome analysis indicates that pals-22 and pals-25 control expression of genes induced not only by natural pathogens of the intestine, but also by natural pathogens of the epidermis. Indeed, in an independent forward genetic screen we identified pals-22 as a repressor and pals-25 as an activator of epidermal defense gene expression. In summary, the species-specific pals-22 and pals-25 genes act as a switch to regulate a program of gene expression, growth, and defense against diverse natural pathogens in C. elegans.

DOI10.1371/journal.ppat.1007528
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/30640956?dopt=Abstract

Alternate JournalPLoS Pathog.
PubMed ID30640956
PubMed Central IDPMC6347328
Grant ListR01 AG052622 / AG / NIA NIH HHS / United States
U19 AI110818 / AI / NIAID NIH HHS / United States