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Cell DOI:10.1016/j.cell.2019.04.009

A High-Throughput Platform to Identify Small-Molecule Inhibitors of CRISPR-Cas9.

Publication TypeJournal Article
Year of Publication2019
AuthorsMaji, B, Gangopadhyay, SA, Lee, M, Shi, M, Wu, P, Heler, R, Mok, B, Lim, D, Siriwardena, SU, Paul, B, Dančík, V, Vetere, A, Mesleh, MF, Marraffini, LA, Liu, DR, Clemons, PA, Wagner, BK, Choudhary, A
Date Published2019 05 02
KeywordsClustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Associated Protein 9, CRISPR-Cas Systems, DNA, Endonucleases, Gene Editing, Genome, High-Throughput Screening Assays, Small Molecule Libraries, Streptococcus pyogenes, Substrate Specificity

The precise control of CRISPR-Cas9 activity is required for a number of genome engineering technologies. Here, we report a generalizable platform that provided the first synthetic small-molecule inhibitors of Streptococcus pyogenes Cas9 (SpCas9) that weigh


Alternate JournalCell
PubMed ID31051099
Grant ListR35 GM118062 / GM / NIGMS NIH HHS / United States
/ HHMI / Howard Hughes Medical Institute / United States
R21 AI126239 / AI / NIAID NIH HHS / United States
RM1 HG009490 / HG / NHGRI NIH HHS / United States