A High-Throughput Platform to Identify Small-Molecule Inhibitors of CRISPR-Cas9.

Cell

Publication type	Journal Article
Authors	Basudeb Maji Soumyashree Gangopadhyay Miseon Lee Mengchao Shi Peng Wu Robert Heler Beverly Mok Donghyun Lim Sachini Siriwardena Bishwajit Paul Vlado Dančík Amedeo Vetere Michael Mesleh Luciano Marraffini David Liu Paul Clemons Bridget Wagner Amit Choudhary
Keywords	DNA Genome Endonucleases CRISPR-Cas Systems Clustered Regularly Interspaced Short Palindromic Repeats High-Throughput Screening Assays Small Molecule Libraries Substrate Specificity Streptococcus pyogenes Gene Editing CRISPR-Associated Protein 9
Abstract	The precise control of CRISPR-Cas9 activity is required for a number of genome engineering technologies. Here, we report a generalizable platform that provided the first synthetic small-molecule inhibitors of Streptococcus pyogenes Cas9 (SpCas9) that weigh
Year of Publication	2019
Journal	Cell
Volume	177
Issue	4
Pages	1067-1079.e19
Date Published	2019 05 02
ISSN	1097-4172
DOI	10.1016/j.cell.2019.04.009
PubMed ID	31051099
Links	DOI Google Scholar PubMed
Grant list	R35 GM118062 / GM / NIGMS NIH HHS / United States HHMI / Howard Hughes Medical Institute / United States R21 AI126239 / AI / NIAID NIH HHS / United States RM1 HG009490 / HG / NHGRI NIH HHS / United States