Scientific Publications

A Microscale Human Liver Platform that Supports the Hepatic Stages of Plasmodium falciparum and vivax.

Publication TypeJournal Article
AuthorsMarch, S., Ng S., Velmurugan S., Galstian A., Shan J., Logan DJ, Carpenter AE, Thomas D., Sim BK, Mota MM, Hoffman SL, and Bhatia SN
AbstractThe Plasmodium liver stage is an attractive target for the development of antimalarial drugs and vaccines, as it provides an opportunity to interrupt the life cycle of the parasite at a critical early stage. However, targeting the liver stage has been difficult. Undoubtedly, a major barrier has been the lack of robust, reliable, and reproducible in vitro liver-stage cultures. Here, we establish the liver stages for both Plasmodium falciparum and Plasmodium vivax in a microscale human liver platform composed of cryopreserved, micropatterned human primary hepatocytes surrounded by supportive stromal cells. Using this system, we have successfully recapitulated the full liver stage of P. falciparum, including the release of infected merozoites and infection of overlaid erythrocytes, as well as the establishment of small forms in late liver stages of P. vivax. Finally, we validate the potential of this platform as a tool for medium-throughput antimalarial drug screening and vaccine development.
Year of Publication2013
JournalCell host & microbe
Volume14
Issue1
Pages104-15
Date Published (YYYY/MM/DD)2013/07/17
ISSN Number1931-3128
DOI10.1016/j.chom.2013.06.005
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/23870318?dopt=Abstract