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Nat Commun DOI:10.1038/ncomms2745

Magnetic barcode assay for genetic detection of pathogens.

Publication TypeJournal Article
Year of Publication2013
AuthorsLiong, M, Hoang, AN, Chung, J, Gural, N, Ford, CB, Min, C, Shah, RR, Ahmad, R, Fernandez-Suarez, M, Fortune, SM, Toner, M, Lee, H, Weissleder, R
JournalNat Commun
Volume4
Pages1752
Date Published2013
ISSN2041-1723
KeywordsBase Sequence, DNA Barcoding, Taxonomic, DNA, Bacterial, Genome, Bacterial, Humans, Magnetics, Molecular Sequence Data, Mutation, Mycobacterium tuberculosis, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Sensitivity and Specificity
Abstract

The task of rapidly identifying patients infected with Mycobacterium tuberculosis in resource-constrained environments remains a challenge. A sensitive and robust platform that does not require bacterial isolation or culture is critical in making informed diagnostic and therapeutic decisions. Here we introduce a platform for the detection of nucleic acids based on a magnetic barcoding strategy. PCR-amplified mycobacterial genes are sequence-specifically captured on microspheres, labelled by magnetic nanoprobes and detected by nuclear magnetic resonance. All components are integrated into a single, small fluidic cartridge for streamlined on-chip operation. We use this platform to detect M. tuberculosis and identify drug-resistance strains from mechanically processed sputum samples within 2.5 h. The specificity of the assay is confirmed by detecting a panel of clinically relevant non-M. tuberculosis bacteria, and the clinical utility is demonstrated by the measurements in M. tuberculosis-positive patient specimens. Combined with portable systems, the magnetic barcode assay holds promise to become a sensitive, high-throughput and low-cost platform for point-of-care diagnostics.

URLhttp://dx.doi.org/10.1038/ncomms2745
DOI10.1038/ncomms2745
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/23612293?dopt=Abstract

Alternate JournalNat Commun
PubMed ID23612293
PubMed Central IDPMC3635151
Grant ListR01-HL113156 / HL / NHLBI NIH HHS / United States
DP2 0D001378 / DP / NCCDPHP CDC HHS / United States
T32 CA079443 / CA / NCI NIH HHS / United States
U19 AI076217 / AI / NIAID NIH HHS / United States
HHSN268201000044C / HL / NHLBI NIH HHS / United States
R01 EB010011 / EB / NIBIB NIH HHS / United States
R01-EB010011 / EB / NIBIB NIH HHS / United States
T32 AI007638 / AI / NIAID NIH HHS / United States
DP2 OD001378 / OD / NIH HHS / United States
T32CA79443 / CA / NCI NIH HHS / United States
R01-EB00462605A1 / EB / NIBIB NIH HHS / United States
R01 EB004626 / EB / NIBIB NIH HHS / United States
HHSN268201000044C / / PHS HHS / United States
R01 HL113156 / HL / NHLBI NIH HHS / United States
P41 EB002503 / EB / NIBIB NIH HHS / United States