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Cell Rep DOI:10.1016/j.celrep.2018.02.081

Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins.

Publication TypeJournal Article
Year of Publication2018
AuthorsJackman, SL, Chen, CH, Chettih, SN, Neufeld, SQ, Drew, IR, Agba, CK, Flaquer, I, Stefano, AN, Kennedy, TJ, Belinsky, JE, Roberston, K, Beron, CC, Sabatini, BL, Harvey, CD, Regehr, WG
JournalCell Rep
Volume22
Issue12
Pages3351-3361
Date Published2018 03 20
ISSN2211-1247
Abstract

Optical methods of interrogating neural circuits have emerged as powerful tools for understanding how the brain drives behaviors. Optogenetic proteins are widely used to control neuronal activity, while genetically encoded fluorescent reporters are used to monitor activity. These proteins are often expressed by injecting viruses, which frequently leads to inconsistent experiments due to misalignment of expression and optical components. Here, we describe how silk fibroin films simplify optogenetic experiments by providing targeted delivery of viruses. Films composed of silk fibroin and virus are applied to the surface of implantable optical components. After surgery, silk releases the virus to transduce nearby cells and provide localized expression around optical fibers and endoscopes. Silk films can also be used to express genetically encoded sensors in large cortical regions by using cranial windows coated with a silk/virus mixture. The ease of use and improved performance provided by silk make this a promising approach for optogenetic studies.

DOI10.1016/j.celrep.2018.02.081
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/29562189?dopt=Abstract

Alternate JournalCell Rep
PubMed ID29562189
PubMed Central IDPMC5894120
Grant ListU01 NS094190 / NS / NINDS NIH HHS / United States
P30 NS072030 / NS / NINDS NIH HHS / United States
R01 MH100568 / MH / NIMH NIH HHS / United States
R35 NS097284 / NS / NINDS NIH HHS / United States
R21 NS093498 / NS / NINDS NIH HHS / United States
R01 NS032405 / NS / NINDS NIH HHS / United States
R01 MH107620 / MH / NIMH NIH HHS / United States
R01 NS089521 / NS / NINDS NIH HHS / United States
F32 NS101889 / NS / NINDS NIH HHS / United States
/ / CIHR / Canada