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Neuron DOI:10.1016/j.neuron.2018.06.002

Layer I Interneurons Sharpen Sensory Maps during Neonatal Development.

Publication TypeJournal Article
Year of Publication2018
AuthorsChe, A, Babij, R, Iannone, AF, Fetcho, RN, Ferrer, M, Liston, C, Fishell, G, García, NVDe Marco
Date Published2018 07 11
KeywordsAnimals, Animals, Newborn, Calcium, Cell Adhesion Molecules, Neuronal, Cerebral Cortex, Extracellular Matrix Proteins, Gene Knockdown Techniques, Interneurons, Mice, Nerve Tissue Proteins, Neural Pathways, Optogenetics, Patch-Clamp Techniques, Physical Stimulation, Pyramidal Cells, Receptors, N-Methyl-D-Aspartate, Receptors, Serotonin, 5-HT3, Reelin Protein, Sensory Deprivation, Serine Endopeptidases, Somatosensory Cortex, Touch, Vibrissae

The neonatal mammal faces an array of sensory stimuli when diverse neuronal types have yet to form sensory maps. How these inputs interact with intrinsic neuronal activity to facilitate circuit assembly is not well understood. By using longitudinal calcium imaging in unanesthetized mouse pups, we show that layer I (LI) interneurons, delineated by co-expression of the 5HT3a serotonin receptor (5HT3aR) and reelin (Re), display spontaneous calcium transients with the highest degree of synchrony among cell types present in the superficial barrel cortex at postnatal day 6 (P6). 5HT3aR Re interneurons are activated by whisker stimulation during this period, and sensory deprivation induces decorrelation of their activity. Moreover, attenuation of thalamic inputs through knockdown of NMDA receptors (NMDARs) in these interneurons results in expansion of whisker responses, aberrant barrel map formation, and deficits in whisker-dependent behavior. These results indicate that recruitment of specific interneuron types during development is critical for adult somatosensory function. VIDEO ABSTRACT.


Alternate JournalNeuron
PubMed ID29937280
PubMed Central IDPMC6152945
Grant ListF30 MH117939 / MH / NIMH NIH HHS / United States
R01 MH109685 / MH / NIMH NIH HHS / United States
R01 MH110553 / MH / NIMH NIH HHS / United States
T32 GM007739 / GM / NIGMS NIH HHS / United States