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Annu Rev Neurosci DOI:10.1146/annurev-neuro-080317-062048

Viral Strategies for Targeting the Central and Peripheral Nervous Systems.

Publication TypeJournal Article
Year of Publication2018
AuthorsBedbrook, CN, Deverman, BE, Gradinaru, V
JournalAnnu Rev Neurosci
Volume41
Pages323-348
Date Published2018 07 08
ISSN1545-4126
Abstract

Recombinant viruses allow for targeted transgene expression in specific cell populations throughout the nervous system. The adeno-associated virus (AAV) is among the most commonly used viruses for neuroscience research. Recombinant AAVs (rAAVs) are highly versatile and can package most cargo composed of desired genes within the capsid's ∼5-kb carrying capacity. Numerous regulatory elements and intersectional strategies have been validated in rAAVs to enable cell type-specific expression. rAAVs can be delivered to specific neuronal populations or globally throughout the animal. The AAV capsids have natural cell type or tissue tropism and trafficking that can be modified for increased specificity. Here, we describe recently engineered AAV capsids and associated cargo that have extended the utility of AAVs in targeting molecularly defined neurons throughout the nervous system, which will further facilitate neuronal circuit interrogation and discovery.

DOI10.1146/annurev-neuro-080317-062048
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/29709207?dopt=Abstract

Alternate JournalAnnu. Rev. Neurosci.
PubMed ID29709207
Grant ListDP2 NS087949 / NS / NINDS NIH HHS / United States
OT2 OD023848 / OD / NIH HHS / United States
R01 AG047664 / AG / NIA NIH HHS / United States
F31 MH102913 / MH / NIMH NIH HHS / United States