Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Engreitz, JM, Pandya-Jones, A, McDonel, P, Shishkin, A, Sirokman, K, Surka, C, Kadri, S, Xing, J, Goren, A, Lander, ES, Plath, K, Guttman, M |
Journal | Science |
Volume | 341 |
Issue | 6147 |
Pages | 1237973 |
Date Published | 2013 Aug 16 |
ISSN | 1095-9203 |
Keywords | Animals, Cell Differentiation, Cell Line, Chromatin, Female, Genome, Male, Mice, Models, Genetic, RNA, Long Noncoding, Transcription, Genetic, X Chromosome, X Chromosome Inactivation |
Abstract | Many large noncoding RNAs (lncRNAs) regulate chromatin, but the mechanisms by which they localize to genomic targets remain unexplored. We investigated the localization mechanisms of the Xist lncRNA during X-chromosome inactivation (XCI), a paradigm of lncRNA-mediated chromatin regulation. During the maintenance of XCI, Xist binds broadly across the X chromosome. During initiation of XCI, Xist initially transfers to distal regions across the X chromosome that are not defined by specific sequences. Instead, Xist identifies these regions by exploiting the three-dimensional conformation of the X chromosome. Xist requires its silencing domain to spread across actively transcribed regions and thereby access the entire chromosome. These findings suggest a model in which Xist coats the X chromosome by searching in three dimensions, modifying chromosome structure, and spreading to newly accessible locations. |
URL | http://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=23828888 |
DOI | 10.1126/science.1237973 |
Pubmed | |
Alternate Journal | Science |
PubMed ID | 23828888 |
PubMed Central ID | PMC3778663 |
Grant List | 1F32GM103139-01 / GM / NIGMS NIH HHS / United States F32 GM103139 / GM / NIGMS NIH HHS / United States DP5OD012190 / OD / NIH HHS / United States P01 GM099134 / GM / NIGMS NIH HHS / United States P50 HG006193 / HG / NHGRI NIH HHS / United States P01GM099134 / GM / NIGMS NIH HHS / United States P50HG006193 / HG / NHGRI NIH HHS / United States DP5 OD012190 / OD / NIH HHS / United States |
Science DOI:10.1126/science.1237973
The Xist lncRNA exploits three-dimensional genome architecture to spread across the X chromosome.
Recent Broad Publications
News at the broad
News / 05.5.22
News / 05.3.22