Field-deployable viral diagnostics using CRISPR-Cas13.

Science
Authors
Cameron Myhrvold
Catherine Freije
Jonathan Gootenberg
Omar Abudayyeh
Hayden Metsky
Ann Durbin
Max Kellner
Amanda Tan
Lauren Paul
Leda Parham
Kimberly Garcia
Kayla Barnes
Bridget Chak
Adriano Mondini
Mauricio Nogueira
Sharon Isern
Scott Michael
Ivette Lorenzana
Nathan Yozwiak
Bronwyn MacInnis
Irene Bosch
Lee Gehrke
Feng Zhang
Pardis Sabeti
Keywords
Humans
Polymorphism, Single Nucleotide
Bacterial Proteins
Endonucleases
Adaptation, Physiological
Dengue
Dengue Virus
CRISPR-Associated Proteins
Enzyme Assays
RNA, Viral
Microcephaly
Zika Virus
Zika Virus Infection
Abstract

Mitigating global infectious disease requires diagnostic tools that are sensitive, specific, and rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) platform can detect Zika virus (ZIKV) and dengue virus (DENV) in patient samples at concentrations as low as 1 copy per microliter. We developed HUDSON (heating unextracted diagnostic samples to obliterate nucleases), a protocol that pairs with SHERLOCK for viral detection directly from bodily fluids, enabling instrument-free DENV detection directly from patient samples in

Year of Publication
2018
Journal
Science
Volume
360
Issue
6387
Pages
444-448
Date Published
2018 04 27
ISSN
1095-9203
DOI
10.1126/science.aas8836
PubMed ID
29700266
PubMed Central ID
PMC6197056
Links
Grant list
R01 AI099210 / AI / NIAID NIH HHS / United States
R01 MH110049 / MH / NIMH NIH HHS / United States
DP1 HL141201 / HL / NHLBI NIH HHS / United States
R33 AI100190 / AI / NIAID NIH HHS / United States
R01 HG009761 / HG / NHGRI NIH HHS / United States
U19 AI110818 / AI / NIAID NIH HHS / United States
R21 AI100190 / AI / NIAID NIH HHS / United States
F30 CA210382 / CA / NCI NIH HHS / United States

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