Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system.
The ability to artificially control transcription is essential both to the study of gene function and to the construction of synthetic gene networks with desired properties. Cas9 is an RNA-guided double-stranded DNA nuclease that participates in the CRISPR-Cas immune defense against prokaryotic viruses. We describe the use of a Cas9 nuclease mutant that retains DNA-binding activity and can be engineered as a programmable transcription repressor by preventing the binding of the RNA polymerase (RNAP) to promoter sequences or as a transcription terminator by blocking the running RNAP. In addition, a fusion between the omega subunit of the RNAP and a Cas9 nuclease mutant directed to bind upstream promoter regions can achieve programmable transcription activation. The simple and efficient modulation of gene expression achieved by this technology is a useful asset for the study of gene networks and for the development of synthetic biology and biotechnological applications.
|Year of Publication
Nucleic Acids Res
|PubMed Central ID
DP2 AI104556 / AI / NIAID NIH HHS / United States
R01 GM044025 / GM / NIGMS NIH HHS / United States
1DP2AI104556-01 / AI / NIAID NIH HHS / United States
DP1MH100706 / DP / NCCDPHP CDC HHS / United States