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Nature methods DOI:10.1038/nmeth.2518

Integrated proteomic analysis of post-translational modifications by serial enrichment.

Publication TypeJournal Article
Year of Publication2013
AuthorsMertins, P, Qiao, JW, Patel, J, Udeshi, ND, Clauser, KR, Mani, DR, Burgess, MW, Gillette, MA, Jaffe, JD, Carr, SA
JournalNature methods
Volume10
Issue7
Pages634-7
Date Published2013/07/01
ISSN1548-7091
Abstract

We report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exemplified by analysis of bortezomib-treated human leukemia cells.

URLhttp://dx.doi.org/10.1038/nmeth.2518
DOI10.1038/nmeth.2518
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/23749302?dopt=Abstract