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Nat Methods DOI:10.1038/nmeth.2518

Integrated proteomic analysis of post-translational modifications by serial enrichment.

Publication TypeJournal Article
Year of Publication2013
AuthorsMertins, P, Qiao, JW, Patel, J, Udeshi, ND, Clauser, KR, Mani, DR, Burgess, MW, Gillette, MA, Jaffe, JD, Carr, SA
JournalNat Methods
Date Published2013 Jul
KeywordsGene Expression Profiling, Mass Spectrometry, Protein Processing, Post-Translational, Proteome, Systems Integration

We report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exemplified by analysis of bortezomib-treated human leukemia cells.


Alternate JournalNat. Methods
PubMed ID23749302
PubMed Central IDPMC3943163
Grant ListU24 CA160034 / CA / NCI NIH HHS / United States
R01HL096738 / HL / NHLBI NIH HHS / United States
R01 HL096738 / HL / NHLBI NIH HHS / United States
HHSN268201000033C / / PHS HHS / United States
HHSN268201000033C / HL / NHLBI NIH HHS / United States
U24CA160034 / CA / NCI NIH HHS / United States