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Nature chemical biology DOI:10.1038/nchembio.1199

Target identification and mechanism of action in chemical biology and drug discovery.

Publication TypeJournal Article
Year of Publication2013
AuthorsSchenone, M, Dančík, V, Wagner, BK, Clemons, PA
JournalNature chemical biology
Volume9
Issue4
Pages232-40
Date Published2013/04/01
ISSN1552-4450
Abstract

Target-identification and mechanism-of-action studies have important roles in small-molecule probe and drug discovery. Biological and technological advances have resulted in the increasing use of cell-based assays to discover new biologically active small molecules. Such studies allow small-molecule action to be tested in a more disease-relevant setting at the outset, but they require follow-up studies to determine the precise protein target or targets responsible for the observed phenotype. Target identification can be approached by direct biochemical methods, genetic interactions or computational inference. In many cases, however, combinations of approaches may be required to fully characterize on-target and off-target effects and to understand mechanisms of small-molecule action.

URLhttp://dx.doi.org/10.1038/nchembio.1199
DOI10.1038/nchembio.1199
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/23508189?dopt=Abstract