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FEMS Microbiol Rev DOI:10.1111/1574-6976.12015

The future is now: single-cell genomics of bacteria and archaea.

Publication TypeJournal Article
Year of Publication2013
AuthorsBlainey, PC
JournalFEMS Microbiol Rev
Date Published2013 May
KeywordsArchaea, Bacteria, Computational Biology, Genetics, Microbial, Genome, Archaeal, Genome, Bacterial, Genomics, High-Throughput Nucleotide Sequencing

Interest in the expanding catalog of uncultivated microorganisms, increasing recognition of heterogeneity among seemingly similar cells, and technological advances in whole-genome amplification and single-cell manipulation are driving considerable progress in single-cell genomics. Here, the spectrum of applications for single-cell genomics, key advances in the development of the field, and emerging methodology for single-cell genome sequencing are reviewed by example with attention to the diversity of approaches and their unique characteristics. Experimental strategies transcending specific methodologies are identified and organized as a road map for future studies in single-cell genomics of environmental microorganisms. Over the next decade, increasingly powerful tools for single-cell genome sequencing and analysis will play key roles in accessing the genomes of uncultivated organisms, determining the basis of microbial community functions, and fundamental aspects of microbial population biology.


Alternate JournalFEMS Microbiol. Rev.
PubMed ID23298390
PubMed Central IDPMC3878092
Grant ListR01 HG004863 / HG / NHGRI NIH HHS / United States