|Publication Type||Journal Article|
|Year of Publication||2013|
|Journal||FEMS microbiology reviews|
Interest in the expanding catalog of uncultivated microbes, increasing recognition of heterogeneity among seemingly similar cells, and technological advances in whole-genome amplification and single cell manipulation are driving considerable progress in single-cell genomics. Here, the spectrum of applications for single-cell genomics, key advances in the development of the field, and emerging methodology for single-cell genome sequencing are reviewed by example with attention to the diversity of approaches and their unique characteristics. Experimental strategies transcending specific methodologies are identified and organized as a roadmap for future studies in single-cell genomics of environmental microbes. Over the next decade, increasingly powerful tools for single-cell genome sequencing and analysis will play key roles in accessing the genomes of uncultivated organisms, determining the basis of microbial community functions at the systems level, and fundamental aspects of microbial population biology. © 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.