Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Blainey, PC |
Journal | FEMS Microbiol Rev |
Volume | 37 |
Issue | 3 |
Pages | 407-27 |
Date Published | 2013 May |
ISSN | 1574-6976 |
Keywords | Archaea, Bacteria, Computational Biology, Genetics, Microbial, Genome, Archaeal, Genome, Bacterial, Genomics, High-Throughput Nucleotide Sequencing |
Abstract | Interest in the expanding catalog of uncultivated microorganisms, increasing recognition of heterogeneity among seemingly similar cells, and technological advances in whole-genome amplification and single-cell manipulation are driving considerable progress in single-cell genomics. Here, the spectrum of applications for single-cell genomics, key advances in the development of the field, and emerging methodology for single-cell genome sequencing are reviewed by example with attention to the diversity of approaches and their unique characteristics. Experimental strategies transcending specific methodologies are identified and organized as a road map for future studies in single-cell genomics of environmental microorganisms. Over the next decade, increasingly powerful tools for single-cell genome sequencing and analysis will play key roles in accessing the genomes of uncultivated organisms, determining the basis of microbial community functions, and fundamental aspects of microbial population biology. |
URL | http://dx.doi.org/10.1111/1574-6976.12015 |
DOI | 10.1111/1574-6976.12015 |
Pubmed | |
Alternate Journal | FEMS Microbiol. Rev. |
PubMed ID | 23298390 |
PubMed Central ID | PMC3878092 |
Grant List | R01 HG004863 / HG / NHGRI NIH HHS / United States |
FEMS Microbiol Rev DOI:10.1111/1574-6976.12015
The future is now: single-cell genomics of bacteria and archaea.
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