Scientific Publications

Differential relationship of DNA replication timing to different forms of human mutation and variation.

Publication TypeJournal Article
AuthorsKoren, A., Polak P., Nemesh J., Michaelson JJ, Sebat J., Sunyaev SR, and McCarroll SA
AbstractHuman genetic variation is distributed nonrandomly across the genome, though the principles governing its distribution are only partially known. DNA replication creates opportunities for mutation, and the timing of DNA replication correlates with the density of SNPs across the human genome. To enable deeper investigation of how DNA replication timing relates to human mutation and variation, we generated a high-resolution map of the human genome's replication timing program and analyzed its relationship to point mutations, copy number variations, and the meiotic recombination hotspots utilized by males and females. DNA replication timing associated with point mutations far more strongly than predicted from earlier analyses and showed a stronger relationship to transversion than transition mutations. Structural mutations arising from recombination-based mechanisms and recombination hotspots used more extensively by females were enriched in early-replicating parts of the genome, though these relationships appeared to relate more strongly to the genomic distribution of causative sequence features. These results indicate differential and sex-specific relationship of DNA replication timing to different forms of mutation and recombination.
Year of Publication2012
JournalAmerican journal of human genetics
Volume91
Issue6
Pages1033-40
Date Published (YYYY/MM/DD)2012/12/07
ISSN Number0002-9297
DOI10.1016/j.ajhg.2012.10.018
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/23176822?dopt=Abstract