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The Journal of infectious diseases DOI:10.1093/infdis/jis679

Whole Genome Pyrosequencing of Rare Hepatitis C Virus Genotypes Enhances Subtype Classification and Identification of Naturally Occurring Drug Resistance Variants.

Publication TypeJournal Article
Year of Publication2012
AuthorsNewman, RM, Kuntzen, T, Weiner, B, Berical, A, Charlebois, P, Kuiken, C, Murphy, DG, Simmonds, P, Bennett, P, Lennon, NJ, Birren, BW, Zody, MC, Allen, TM, Henn, MR
JournalThe Journal of infectious diseases
Date Published2012/11/06

Background. Infection with hepatitis C virus (HCV) is a burgeoning worldwide public health problem, with 170 million infected individuals and an estimated 20 million deaths in the coming decades. While six main genotypes generally distinguish the global geographic diversity of HCV, a multitude of closely related subtypes within these genotypes are poorly defined and may influence clinical outcome and treatment options. Unfortunately, the paucity of genetic data from many of these subtypes makes time-consuming primer walking the limiting step for sequencing understudied subtypes.Methods. Here we combined long-range PCR amplification with pyrosequencing for a rapid approach to generate the complete viral coding region of 31 samples representing poorly defined HCV subtypes.Results. Phylogenetic classification based on full genome sequences validated previously identified HCV subtypes, identified a recombinant sequence and a new distinct subtype of genotype 4. Unlike conventional sequencing methods, use of deep sequencing also facilitated characterization of minor drug resistance variants within these uncommon or, in some cases, previously uncharacterized HCV subtypes.Conclusions. These data aid in classification of uncommon HCV subtypes while also providing a high-resolution view of viral diversity within infected patients, which may be relevant to the development of therapeutic regimens to minimize drug resistance.