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Genome Biol DOI:10.1186/gb-2012-13-10-r92

Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling.

Publication TypeJournal Article
Year of Publication2012
AuthorsBoyle, P, Clement, K, Gu, H, Smith, ZD, Ziller, M, Fostel, JL, Holmes, L, Meldrim, J, Kelley, F, Gnirke, A, Meissner, A
JournalGenome Biol
Volume13
Issue10
PagesR92
Date Published2012 Oct 03
ISSN1474-760X
KeywordsAnimals, CpG Islands, DNA Methylation, Genome, Humans, Mice, Molecular Sequence Data, NIH 3T3 Cells, Sequence Analysis, DNA, Sulfites
Abstract

Sequencing-based approaches have led to new insights about DNA methylation. While many different techniques for genome-scale mapping of DNA methylation have been employed, throughput has been a key limitation for most. To further facilitate the mapping of DNA methylation, we describe a protocol for gel-free multiplexed reduced representation bisulfite sequencing (mRRBS) that reduces the workload dramatically and enables processing of 96 or more samples per week. mRRBS achieves similar CpG coverage to the original RRBS protocol, while the higher throughput and lower cost make it better suited for large-scale DNA methylation mapping studies, including cohorts of cancer samples.

URLhttp://genomebiology.com/content/13/10/R92
DOI10.1186/gb-2012-13-10-r92
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/23034176?dopt=Abstract

Alternate JournalGenome Biol.
PubMed ID23034176
PubMed Central IDPMC3491420
Grant ListU01ES017155 / ES / NIEHS NIH HHS / United States