Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling.

Genome Biol
Authors
Keywords
Abstract

Sequencing-based approaches have led to new insights about DNA methylation. While many different techniques for genome-scale mapping of DNA methylation have been employed, throughput has been a key limitation for most. To further facilitate the mapping of DNA methylation, we describe a protocol for gel-free multiplexed reduced representation bisulfite sequencing (mRRBS) that reduces the workload dramatically and enables processing of 96 or more samples per week. mRRBS achieves similar CpG coverage to the original RRBS protocol, while the higher throughput and lower cost make it better suited for large-scale DNA methylation mapping studies, including cohorts of cancer samples.

Year of Publication
2012
Journal
Genome Biol
Volume
13
Issue
10
Pages
R92
Date Published
2012 Oct 03
ISSN
1474-760X
URL
DOI
10.1186/gb-2012-13-10-r92
PubMed ID
23034176
PubMed Central ID
PMC3491420
Links
Grant list
U01ES017155 / ES / NIEHS NIH HHS / United States