Scientific Publications

Mapping the hallmarks of lung adenocarcinoma with massively parallel sequencing.

Publication TypeJournal Article
AuthorsImielinski, M., Berger AH, Hammerman PS, Hernandez B., Pugh TJ, Hodis E., Cho J., Sivachenko A., Sougnez C., Auclair D., Lawrence MS, Stojanov P., Cibulskis K., Sharifnia T., Greulich H., Banerji S., Zander T., Thompson K., Winckler W., Kwiatkowski D., Johnson B. E., Jänne PA, Pao W., Travis WD, Gabriel SB, Lander E. S., Thomas RK, Garraway LA, Getz G., and Meyerson M.
AbstractLung adenocarcinoma, the most common subtype of non-small cell lung cancer, is responsible for more than 500,000 deaths per year worldwide. Here, we report exome and genome sequences of 183 lung adenocarcinoma tumor/normal DNA pairs. These analyses revealed a mean exonic somatic mutation rate of 12.0 events/megabase and identified the majority of genes previously reported as significantly mutated in lung adenocarcinoma. In addition, we identified statistically recurrent somatic mutations in the splicing factor gene U2AF1 and truncating mutations affecting RBM10 and ARID1A. Analysis of nucleotide context-specific mutation signatures grouped the sample set into distinct clusters that correlated with smoking history and alterations of reported lung adenocarcinoma genes. Whole-genome sequence analysis revealed frequent structural rearrangements, including in-frame exonic alterations within EGFR and SIK2 kinases. The candidate genes identified in this study are attractive targets for biological characterization and therapeutic targeting of lung adenocarcinoma.
Year of Publication2012
JournalCell
Volume150
Issue6
Pages1107-20
Date Published (YYYY/MM/DD)2012/09/14
ISSN Number0092-8674
DOI10.1016/j.cell.2012.08.029
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/22980975?dopt=Abstract