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Nat Genet DOI:10.1038/ng.2382

Mouse ooplasm confers context-specific reprogramming capacity.

Publication TypeJournal Article
Year of Publication2012
AuthorsChan, MM, Smith, ZD, Egli, D, Regev, A, Meissner, A
JournalNat Genet
Volume44
Issue9
Pages978-80
Date Published2012 Sep
ISSN1546-1718
KeywordsAnimals, Cytoplasm, DNA Methylation, Embryo, Mammalian, Embryonic Development, Female, Male, Mice, Mice, Inbred C57BL, Mice, Inbred DBA, Nuclear Transfer Techniques, Oocytes, Organ Specificity, Promoter Regions, Genetic, Sperm-Ovum Interactions
Abstract

Enucleated oocytes have the distinctive ability to reprogram somatic nuclei back to totipotency. Here, we investigate genome-scale DNA methylation patterns after nuclear transfer and compare them to the dynamics at fertilization. We identify specific targets for DNA demethylation after nuclear transfer, such as germline-associated promoters, as well as unique limitations that include certain repetitive element classes.

URLhttp://dx.doi.org/10.1038/ng.2382
DOI10.1038/ng.2382
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/22902786?dopt=Abstract

Alternate JournalNat. Genet.
PubMed ID22902786
PubMed Central IDPMC3432711
Grant ListP01 GM099117 / GM / NIGMS NIH HHS / United States
U01ES017155 / ES / NIEHS NIH HHS / United States
5DP1OD003958 / OD / NIH HHS / United States
DP1 CA174427 / CA / NCI NIH HHS / United States
P01GM099117 / GM / NIGMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States
P50 HG006193 / HG / NHGRI NIH HHS / United States
U01 ES017155 / ES / NIEHS NIH HHS / United States
DP1 OD003958 / OD / NIH HHS / United States
1P50HG006193 / HG / NHGRI NIH HHS / United States