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Nat Genet DOI:10.1038/ng.2382

Mouse ooplasm confers context-specific reprogramming capacity.

Publication TypeJournal Article
Year of Publication2012
AuthorsChan, MM, Smith, ZD, Egli, D, Regev, A, Meissner, A
JournalNat Genet
Date Published2012 Sep
KeywordsAnimals, Cytoplasm, DNA Methylation, Embryo, Mammalian, Embryonic Development, Female, Male, Mice, Mice, Inbred C57BL, Mice, Inbred DBA, Nuclear Transfer Techniques, Oocytes, Organ Specificity, Promoter Regions, Genetic, Sperm-Ovum Interactions

Enucleated oocytes have the distinctive ability to reprogram somatic nuclei back to totipotency. Here, we investigate genome-scale DNA methylation patterns after nuclear transfer and compare them to the dynamics at fertilization. We identify specific targets for DNA demethylation after nuclear transfer, such as germline-associated promoters, as well as unique limitations that include certain repetitive element classes.


Alternate JournalNat. Genet.
PubMed ID22902786
PubMed Central IDPMC3432711
Grant ListP01 GM099117 / GM / NIGMS NIH HHS / United States
U01ES017155 / ES / NIEHS NIH HHS / United States
5DP1OD003958 / OD / NIH HHS / United States
DP1 CA174427 / CA / NCI NIH HHS / United States
P01GM099117 / GM / NIGMS NIH HHS / United States
/ / Howard Hughes Medical Institute / United States
P50 HG006193 / HG / NHGRI NIH HHS / United States
U01 ES017155 / ES / NIEHS NIH HHS / United States
DP1 OD003958 / OD / NIH HHS / United States
1P50HG006193 / HG / NHGRI NIH HHS / United States