Finished bacterial genomes from shotgun sequence data.

Genome Res
Authors
Keywords
Abstract

Exceptionally accurate genome reference sequences have proven to be of great value to microbial researchers. Thus, to date, about 1800 bacterial genome assemblies have been "finished" at great expense with the aid of manual laboratory and computational processes that typically iterate over a period of months or even years. By applying a new laboratory design and new assembly algorithm to 16 samples, we demonstrate that assemblies exceeding finished quality can be obtained from whole-genome shotgun data and automated computation. Cost and time requirements are thus dramatically reduced.

Year of Publication
2012
Journal
Genome Res
Volume
22
Issue
11
Pages
2270-7
Date Published
2012 Nov
ISSN
1549-5469
URL
DOI
10.1101/gr.141515.112
PubMed ID
22829535
PubMed Central ID
PMC3483556
Links
Grant list
HHSN272200900018C / AI / NIAID NIH HHS / United States
U54 HG003067 / HG / NHGRI NIH HHS / United States
R01 HG003474 / HG / NHGRI NIH HHS / United States
U54HG003067 / HG / NHGRI NIH HHS / United States
R01HG003474 / HG / NHGRI NIH HHS / United States