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Genome Res DOI:10.1101/gr.141515.112

Finished bacterial genomes from shotgun sequence data.

Publication TypeJournal Article
Year of Publication2012
AuthorsRibeiro, FJ, Przybylski, D, Yin, S, Sharpe, T, Gnerre, S, Abouelleil, A, Berlin, AM, Montmayeur, A, Shea, TP, Walker, BJ, Young, SK, Russ, C, Nusbaum, C, MacCallum, I, Jaffe, DB
JournalGenome Res
Date Published2012 Nov
KeywordsAlgorithms, Bacteria, Genome, Bacterial, Genomic Library, Sequence Analysis, DNA

Exceptionally accurate genome reference sequences have proven to be of great value to microbial researchers. Thus, to date, about 1800 bacterial genome assemblies have been "finished" at great expense with the aid of manual laboratory and computational processes that typically iterate over a period of months or even years. By applying a new laboratory design and new assembly algorithm to 16 samples, we demonstrate that assemblies exceeding finished quality can be obtained from whole-genome shotgun data and automated computation. Cost and time requirements are thus dramatically reduced.


Alternate JournalGenome Res.
PubMed ID22829535
PubMed Central IDPMC3483556
Grant ListHHSN272200900018C / AI / NIAID NIH HHS / United States
U54 HG003067 / HG / NHGRI NIH HHS / United States
R01 HG003474 / HG / NHGRI NIH HHS / United States
U54HG003067 / HG / NHGRI NIH HHS / United States
R01HG003474 / HG / NHGRI NIH HHS / United States