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Genome research DOI:10.1101/gr.141515.112

Finished bacterial genomes from shotgun sequence data.

Publication TypeJournal Article
Year of Publication2012
AuthorsRibeiro, F, Przybylski, D, Yin, S, Sharpe, T, Gnerre, S, Abouelleil, A, Berlin, AM, Montmayeur, A, Shea, TP, Walker, BJ, Young, SK, Russ, C, Maccallum, I, Nusbaum, C, Jaffe, DB
JournalGenome research
Date Published2012/07/24

Exceptionally accurate genome reference sequences have proven to be of great value to microbial researchers. Thus, to date about 1800 bacterial genome assemblies have been "finished" at great expense with the aid of manual laboratory and computational processes that typically iterate over a period of months or even years. By applying a new laboratory design and new assembly algorithm to sixteen samples, we demonstrate that assemblies exceeding finished quality can be obtained from whole-genome shotgun data and automated computation. Cost and time requirements are thus dramatically reduced.