You are here

Genome Res DOI:10.1101/gr.138925.112

Paired-end sequencing of Fosmid libraries by Illumina.

Publication TypeJournal Article
Year of Publication2012
AuthorsWilliams, LJS, Tabbaa, DG, Li, N, Berlin, AM, Shea, TP, MacCallum, I, Lawrence, MS, Drier, Y, Getz, G, Young, SK, Jaffe, DB, Nusbaum, C, Gnirke, A
JournalGenome Res
Date Published2012 Nov
KeywordsAnimals, Escherichia coli, Gene Rearrangement, Genetic Vectors, Genome, Bacterial, Genome, Fungal, Genomic Library, Mice, Mice, Inbred C57BL, Schizosaccharomyces, Sequence Analysis, DNA

Eliminating the bacterial cloning step has been a major factor in the vastly improved efficiency of massively parallel sequencing approaches. However, this also has made it a technical challenge to produce the modern equivalent of the Fosmid- or BAC-end sequences that were crucial for assembling and analyzing complex genomes during the Sanger-based sequencing era. To close this technology gap, we developed Fosill, a method for converting Fosmids to Illumina-compatible jumping libraries. We constructed Fosmid libraries in vectors with Illumina primer sequences and specific nicking sites flanking the cloning site. Our family of pFosill vectors allows multiplex Fosmid cloning of end-tagged genomic fragments without physical size selection and is compatible with standard and multiplex paired-end Illumina sequencing. To excise the bulk of each cloned insert, we introduced two nicks in the vector, translated them into the inserts, and cleaved them. Recircularization of the vector via coligation of insert termini followed by inverse PCR generates a jumping library for paired-end sequencing with 101-base reads. The yield of unique Fosmid-sized jumps is sufficiently high, and the background of short, incorrectly spaced and chimeric artifacts sufficiently low, to enable applications such as mapping of structural variation and scaffolding of de novo assemblies. We demonstrate the power of Fosill to map genome rearrangements in a cancer cell line and identified three fusion genes that were corroborated by RNA-seq data. Our Fosill-powered assembly of the mouse genome has an N50 scaffold length of 17.0 Mb, rivaling the connectivity (16.9 Mb) of the Sanger-sequencing based draft assembly.


Alternate JournalGenome Res.
PubMed ID22800726
PubMed Central IDPMC3483553
Grant ListHG003067-05 / HG / NHGRI NIH HHS / United States
HHSN272200900018C / AI / NIAID NIH HHS / United States
U54 HG003067 / HG / NHGRI NIH HHS / United States
R01 HG003474 / HG / NHGRI NIH HHS / United States
HG003067-10 / HG / NHGRI NIH HHS / United States