Scientific Publications

Strain-Specific Regulation of Striatal Phenotype in Drd2-eGFP BAC Transgenic Mice.

Publication TypeJournal Article
AuthorsChan, CS, Peterson JD, Gertler TS, Glajch KE, Quintana RE, Cui Q., Sebel LE, Plotkin JL, Shen W., Heiman M., Heintz N., Greengard P., and Surmeier DJ
AbstractMice carrying bacterial artificial chromosome (BAC) transgenes have become important tools for neuroscientists, providing a powerful means of dissecting complex neural circuits in the brain. Recently, it was reported that one popular line of these mice-mice possessing a BAC transgene with a D(2) dopamine receptor (Drd2) promoter construct coupled to an enhanced green fluorescent protein (eGFP) reporter-had abnormal striatal gene expression, physiology, and motor behavior. Unlike most of the work using BAC mice, this interesting study relied upon mice backcrossed on the outbred Swiss Webster (SW) strain that were homozygous for the Drd2-eGFP BAC transgene. The experiments reported here were conducted to determine whether mouse strain or zygosity was a factor in the reported abnormalities. As reported, SW mice were very sensitive to transgene expression. However, in more commonly used inbred strains of mice (C57BL/6, FVB/N) that were hemizygous for the transgene, the Drd2-eGFP BAC transgene did not alter striatal gene expression, physiology, or motor behavior. Thus, the use of inbred strains of mice that are hemizygous for the Drd2 BAC transgene provides a reliable tool for studying basal ganglia function.
Year of Publication2012
JournalThe Journal of neuroscience : the official journal of the Society for Neuroscience
Volume32
Issue27
Pages9124-9132
Date Published (YYYY/MM/DD)2012/07/04
ISSN Number0270-6474
DOI10.1523/JNEUROSCI.0229-12.2012
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/22764222?dopt=Abstract