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Genetics DOI:10.1534/genetics.112.141200

Activity-dependent A-to-I RNA Editing in Rat Cortical Neurons.

Publication TypeJournal Article
Year of Publication2012
AuthorsSanjana, NE, Levanon, EY, Hueske, EA, Ambrose, JM, Li, JB
JournalGenetics
Date Published2012/06/19
ISSN0016-6731
Abstract

Changes in neural activity influence synaptic plasticity/scaling, gene expression, and epigenetic modifications. We present the first evidence that short-term and persistent changes in neural activity can alter adenosine-to-inosine (A-to-I) RNA editing, a post transcriptional site-specific modification found in several neuron-specific transcripts. In rat cortical neuron cultures, activity-dependent changes in A-to-I RNA editing in coding exons are present after 6 hours of high potassium depolarization but not after 1 hour and require calcium entry into neurons. When treatments are extended from hours to days, we observe a negative feedback phenomenon: Chronic depolarization increases editing at many sites and chronic silencing decreases editing. We present several different modulations of neural activity that change the expression of different mRNA isoforms through editing.

URLhttp://www.genetics.org/cgi/pmidlookup?view=long&pmid=22714409
DOI10.1534/genetics.112.141200
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/22714409?dopt=Abstract