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Genetics DOI:10.1534/genetics.112.141200

Activity-dependent A-to-I RNA editing in rat cortical neurons.

Publication TypeJournal Article
Year of Publication2012
AuthorsSanjana, NE, Levanon, EY, Hueske, EA, Ambrose, JM, Li, JBilly
Date Published2012 Sep
KeywordsAdenosine, Animals, Calcium, Cerebral Cortex, Inosine, Membrane Potentials, Neurons, Rats, RNA Editing, RNA, Messenger, Synapses, Time Factors

Changes in neural activity influence synaptic plasticity/scaling, gene expression, and epigenetic modifications. We present the first evidence that short-term and persistent changes in neural activity can alter adenosine-to-inosine (A-to-I) RNA editing, a post-transcriptional site-specific modification found in several neuron-specific transcripts. In rat cortical neuron cultures, activity-dependent changes in A-to-I RNA editing in coding exons are present after 6 hr of high potassium depolarization but not after 1 hr and require calcium entry into neurons. When treatments are extended from hours to days, we observe a negative feedback phenomenon: Chronic depolarization increases editing at many sites and chronic silencing decreases editing. We present several different modulations of neural activity that change the expression of different mRNA isoforms through editing.


Alternate JournalGenetics
PubMed ID22714409
PubMed Central IDPMC3430542
Grant List / / Howard Hughes Medical Institute / United States