Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Sanjana, NE, Levanon, EY, Hueske, EA, Ambrose, JM, Li, JBilly |
Journal | Genetics |
Volume | 192 |
Issue | 1 |
Pages | 281-7 |
Date Published | 2012 Sep |
ISSN | 1943-2631 |
Keywords | Adenosine, Animals, Calcium, Cerebral Cortex, Inosine, Membrane Potentials, Neurons, Rats, RNA Editing, RNA, Messenger, Synapses, Time Factors |
Abstract | Changes in neural activity influence synaptic plasticity/scaling, gene expression, and epigenetic modifications. We present the first evidence that short-term and persistent changes in neural activity can alter adenosine-to-inosine (A-to-I) RNA editing, a post-transcriptional site-specific modification found in several neuron-specific transcripts. In rat cortical neuron cultures, activity-dependent changes in A-to-I RNA editing in coding exons are present after 6 hr of high potassium depolarization but not after 1 hr and require calcium entry into neurons. When treatments are extended from hours to days, we observe a negative feedback phenomenon: Chronic depolarization increases editing at many sites and chronic silencing decreases editing. We present several different modulations of neural activity that change the expression of different mRNA isoforms through editing. |
URL | http://www.genetics.org/cgi/pmidlookup?view=long&pmid=22714409 |
DOI | 10.1534/genetics.112.141200 |
Pubmed | |
Alternate Journal | Genetics |
PubMed ID | 22714409 |
PubMed Central ID | PMC3430542 |
Grant List | / / Howard Hughes Medical Institute / United States |