|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Sanjana, NE, Levanon, EY, Hueske, EA, Ambrose, JM, Li, JBilly|
|Date Published||2012 Sep|
|Keywords||Adenosine, Animals, Calcium, Cerebral Cortex, Inosine, Membrane Potentials, Neurons, Rats, RNA Editing, RNA, Messenger, Synapses, Time Factors|
Changes in neural activity influence synaptic plasticity/scaling, gene expression, and epigenetic modifications. We present the first evidence that short-term and persistent changes in neural activity can alter adenosine-to-inosine (A-to-I) RNA editing, a post-transcriptional site-specific modification found in several neuron-specific transcripts. In rat cortical neuron cultures, activity-dependent changes in A-to-I RNA editing in coding exons are present after 6 hr of high potassium depolarization but not after 1 hr and require calcium entry into neurons. When treatments are extended from hours to days, we observe a negative feedback phenomenon: Chronic depolarization increases editing at many sites and chronic silencing decreases editing. We present several different modulations of neural activity that change the expression of different mRNA isoforms through editing.
|PubMed Central ID||PMC3430542|
|Grant List||/ / Howard Hughes Medical Institute / United States|