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Genetics DOI:10.1534/genetics.112.141200

Activity-dependent A-to-I RNA Editing in Rat Cortical Neurons.

Publication TypeJournal Article
Year of Publication2012
AuthorsSanjana, NE, Levanon, EY, Hueske, EA, Ambrose, JM, Li, JB
Date Published2012/06/19

Changes in neural activity influence synaptic plasticity/scaling, gene expression, and epigenetic modifications. We present the first evidence that short-term and persistent changes in neural activity can alter adenosine-to-inosine (A-to-I) RNA editing, a post transcriptional site-specific modification found in several neuron-specific transcripts. In rat cortical neuron cultures, activity-dependent changes in A-to-I RNA editing in coding exons are present after 6 hours of high potassium depolarization but not after 1 hour and require calcium entry into neurons. When treatments are extended from hours to days, we observe a negative feedback phenomenon: Chronic depolarization increases editing at many sites and chronic silencing decreases editing. We present several different modulations of neural activity that change the expression of different mRNA isoforms through editing.