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Science DOI:10.1126/science.1218595

Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation.

Publication TypeJournal Article
Year of Publication2012
AuthorsJain, M, Nilsson, R, Sharma, S, Madhusudhan, N, Kitami, T, Souza, AL, Kafri, R, Kirschner, MW, Clish, CB, Mootha, VK
Date Published2012 May 25
KeywordsBreast Neoplasms, Cell Cycle, Cell Line, Cell Line, Tumor, Cell Proliferation, Cell Transformation, Neoplastic, Chromatography, Liquid, Culture Media, Gene Expression, Gene Expression Profiling, Glycine, Humans, Metabolic Networks and Pathways, Metabolome, Mitochondria, Neoplasms, Purines, Tandem Mass Spectrometry

Metabolic reprogramming has been proposed to be a hallmark of cancer, yet a systematic characterization of the metabolic pathways active in transformed cells is currently lacking. Using mass spectrometry, we measured the consumption and release (CORE) profiles of 219 metabolites from media across the NCI-60 cancer cell lines, and integrated these data with a preexisting atlas of gene expression. This analysis identified glycine consumption and expression of the mitochondrial glycine biosynthetic pathway as strongly correlated with rates of proliferation across cancer cells. Antagonizing glycine uptake and its mitochondrial biosynthesis preferentially impaired rapidly proliferating cells. Moreover, higher expression of this pathway was associated with greater mortality in breast cancer patients. Increased reliance on glycine may represent a metabolic vulnerability for selectively targeting rapid cancer cell proliferation.


Alternate JournalScience
PubMed ID22628656
PubMed Central IDPMC3526189
Grant ListR01 GM026875 / GM / NIGMS NIH HHS / United States
K08HL107451 / HL / NHLBI NIH HHS / United States
K08 HL107451 / HL / NHLBI NIH HHS / United States
R01 DK081457 / DK / NIDDK NIH HHS / United States
R01DK081457 / DK / NIDDK NIH HHS / United States