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Proc Natl Acad Sci U S A DOI:10.1073/pnas.1119540109

RNA signatures allow rapid identification of pathogens and antibiotic susceptibilities.

Publication TypeJournal Article
Year of Publication2012
AuthorsBarczak, AK, Gomez, JE, Kaufmann, BB, Hinson, ER, Cosimi, L, Borowsky, ML, Onderdonk, AB, Stanley, SA, Kaur, D, Bryant, KF, Knipe, DM, Sloutsky, A, Hung, DT
JournalProc Natl Acad Sci U S A
Volume109
Issue16
Pages6217-22
Date Published2012 Apr 17
ISSN1091-6490
KeywordsAnti-Bacterial Agents, Bacteria, Cells, Cultured, Erythrocytes, Fungi, HEK293 Cells, HeLa Cells, Herpesvirus 1, Human, Herpesvirus 2, Human, Humans, Microbial Sensitivity Tests, Plasmodium falciparum, Reproducibility of Results, RNA, Species Specificity, Urine
Abstract

With rising rates of drug-resistant infections, there is a need for diagnostic methods that rapidly can detect the presence of pathogens and reveal their susceptibility to antibiotics. Here we propose an approach to diagnosing the presence and drug-susceptibility of infectious diseases based on direct detection of RNA from clinical samples. We demonstrate that species-specific RNA signatures can be used to identify a broad spectrum of infectious agents, including bacteria, viruses, yeast, and parasites. Moreover, we show that the behavior of a small set of bacterial transcripts after a brief antibiotic pulse can rapidly differentiate drug-susceptible and -resistant organisms and that these measurements can be made directly from clinical materials. Thus, transcriptional signatures could form the basis of a uniform diagnostic platform applicable across a broad range of infectious agents.

URLhttp://www.pnas.org/cgi/pmidlookup?view=long&pmid=22474362
DOI10.1073/pnas.1119540109
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/22474362?dopt=Abstract

Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID22474362
PubMed Central IDPMC3341018
Grant ListT32 AI007061 / AI / NIAID NIH HHS / United States
K08 AI080944 / AI / NIAID NIH HHS / United States
F32 AI081477 / AI / NIAID NIH HHS / United States
R01 AI063106 / AI / NIAID NIH HHS / United States
U54 AI057159 / AI / NIAID NIH HHS / United States
AI 063106 / AI / NIAID NIH HHS / United States
AI 081477 / AI / NIAID NIH HHS / United States