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Sonic hedgehog expression in corticofugal projection neurons directs cortical microcircuit formation.
|Publication Type||Journal Article|
|Authors||Harwell, CC, Parker PR, Gee SM, Okada A., McConnell SK, Kreitzer AC, and Kriegstein AR|
|Abstract||The precise connectivity of inputs and outputs is critical for cerebral cortex function; however, the cellular mechanisms that establish these connections are poorly understood. Here, we show that the secreted molecule Sonic Hedgehog (Shh) is involved in synapse formation of a specific cortical circuit. Shh is expressed in layer V corticofugal projection neurons and the Shh receptor, Brother of CDO (Boc), is expressed in local and callosal projection neurons of layer II/III that synapse onto the subcortical projection neurons. Layer V neurons of mice lacking functional Shh exhibit decreased synapses. Conversely, the loss of functional Boc leads to a reduction in the strength of synaptic connections onto layer Vb, but not layer II/III, pyramidal neurons. These results demonstrate that Shh is expressed in postsynaptic target cells while Boc is expressed in a complementary population of presynaptic input neurons, and they function to guide the formation of cortical microcircuitry. VIDEO ABSTRACT:|
|Year of Publication||2012|
|Date Published (YYYY/MM/DD)||2012/03/22|