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Nature DOI:10.1038/nature22312

Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations.

Publication TypeJournal Article
Year of Publication2017
AuthorsMerkle, FT, Ghosh, S, Kamitaki, N, Mitchell, J, Avior, Y, Mello, C, Kashin, S, Mekhoubad, S, Ilic, D, Charlton, M, Saphier, G, Handsaker, RE, Genovese, G, Bar, S, Benvenisty, N, McCarroll, SA, Eggan, K
JournalNature
Volume545
Issue7653
Pages229-233
Date Published2017 05 11
ISSN1476-4687
KeywordsAlleles, Cell Count, Cell Differentiation, Cell Division, Cell Line, DNA, DNA Mutational Analysis, Exome, Genes, Dominant, Genes, p53, Human Embryonic Stem Cells, Humans, Induced Pluripotent Stem Cells, Loss of Heterozygosity, Mosaicism, Mutation, Neoplasms, Protein Domains, Selection, Genetic, Tumor Suppressor Protein p53
Abstract

Human pluripotent stem cells (hPS cells) can self-renew indefinitely, making them an attractive source for regenerative therapies. This expansion potential has been linked with the acquisition of large copy number variants that provide mutated cells with a growth advantage in culture. The nature, extent and functional effects of other acquired genome sequence mutations in cultured hPS cells are not known. Here we sequence the protein-coding genes (exomes) of 140 independent human embryonic stem cell (hES cell) lines, including 26 lines prepared for potential clinical use. We then apply computational strategies for identifying mutations present in a subset of cells in each hES cell line. Although such mosaic mutations were generally rare, we identified five unrelated hES cell lines that carried six mutations in the TP53 gene that encodes the tumour suppressor P53. The TP53 mutations we observed are dominant negative and are the mutations most commonly seen in human cancers. We found that the TP53 mutant allelic fraction increased with passage number under standard culture conditions, suggesting that the P53 mutations confer selective advantage. We then mined published RNA sequencing data from 117 hPS cell lines, and observed another nine TP53 mutations, all resulting in coding changes in the DNA-binding domain of P53. In three lines, the allelic fraction exceeded 50%, suggesting additional selective advantage resulting from the loss of heterozygosity at the TP53 locus. As the acquisition and expansion of cancer-associated mutations in hPS cells may go unnoticed during most applications, we suggest that careful genetic characterization of hPS cells and their differentiated derivatives be carried out before clinical use.

DOI10.1038/nature22312
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/28445466?dopt=Abstract

Alternate JournalNature
PubMed ID28445466
PubMed Central IDPMC5427175
Grant ListP01 GM099117 / GM / NIGMS NIH HHS / United States
SBF001\1016 / / Academy of Medical Sciences / United Kingdom
R01 HG006855 / HG / NHGRI NIH HHS / United States
R01 HL109525 / HL / NHLBI NIH HHS / United States
5K99NS08371 / NH / NIH HHS / United States
/ / Wellcome Trust / United Kingdom