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Genome Res DOI:10.1101/gr.128124.111

Cost-effective, high-throughput DNA sequencing libraries for multiplexed target capture.

Publication TypeJournal Article
Year of Publication2012
AuthorsRohland, N, Reich, D
JournalGenome Res
Volume22
Issue5
Pages939-46
Date Published2012 May
ISSN1549-5469
KeywordsCost-Benefit Analysis, DNA Cleavage, DNA Probes, Escherichia coli, Gene Library, Genome, Bacterial, Genome, Human, High-Throughput Nucleotide Sequencing, Humans, Male, Prostatic Neoplasms, Sequence Analysis, DNA
Abstract

Improvements in technology have reduced the cost of DNA sequencing to the point that the limiting factor for many experiments is the time and reagent cost of sample preparation. We present an approach in which 192 sequencing libraries can be produced in a single day of technician time at a cost of about $15 per sample. These libraries are effective not only for low-pass whole-genome sequencing, but also for simultaneously enriching them in pools of approximately 100 individually barcoded samples for a subset of the genome without substantial loss in efficiency of target capture. We illustrate the power and effectiveness of this approach on about 2000 samples from a prostate cancer study.

URLhttp://www.genome.cshlp.org/cgi/pmidlookup?view=long&pmid=22267522
DOI10.1101/gr.128124.111
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/22267522?dopt=Abstract

Alternate JournalGenome Res.
PubMed ID22267522
PubMed Central IDPMC3337438
Grant ListR01 CA129435 / CA / NCI NIH HHS / United States
HG004726 / HG / NHGRI NIH HHS / United States
CA63464 / CA / NCI NIH HHS / United States
CA129435 / CA / NCI NIH HHS / United States
R01 HL084107 / HL / NHLBI NIH HHS / United States
R01 CA063464 / CA / NCI NIH HHS / United States
U01 CA063464 / CA / NCI NIH HHS / United States
HL084107 / HL / NHLBI NIH HHS / United States
U01 HG004726 / HG / NHGRI NIH HHS / United States