Scientific Publications

STK33 kinase inhibitor BRD-8899 has no effect on KRAS-dependent cancer cell viability.

Publication TypeJournal Article
AuthorsLuo, T., Masson K., Jaffe JD, Silkworth W., Ross NT, Scherer CA, Scholl C., Fröhling S., Carr SA, Stern AM, Schreiber SL, and Golub T. R.
AbstractApproximately 30% of human cancers harbor oncogenic gain-of-function mutations in KRAS. Despite interest in KRAS as a therapeutic target, direct blockade of KRAS function with small molecules has yet to be demonstrated. Based on experiments that lower mRNA levels of protein kinases, KRAS-dependent cancer cells were proposed to have a unique requirement for the serine/threonine kinase STK33. Thus, it was suggested that small-molecule inhibitors of STK33 might have therapeutic benefit in these cancers. Here, we describe the development of selective, low nanomolar inhibitors of STK33's kinase activity. The most potent and selective of these, BRD8899, failed to kill KRAS-dependent cells. While several explanations for this result exist, our data are most consistent with the view that inhibition of STK33's kinase activity does not represent a promising anti-KRAS therapeutic strategy.
Year of Publication2012
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue8
Pages2860-5
Date Published (YYYY/MM/DD)2012/02/21
ISSN Number0027-8424
DOI10.1073/pnas.1120589109
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/22323609?dopt=Abstract