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Cell Host Microbe DOI:10.1016/j.chom.2011.08.005

The genome of th17 cell-inducing segmented filamentous bacteria reveals extensive auxotrophy and adaptations to the intestinal environment.

Publication TypeJournal Article
Year of Publication2011
AuthorsSczesnak, A, Segata, N, Qin, X, Gevers, D, Petrosino, JF, Huttenhower, C, Littman, DR, Ivanov, II
JournalCell Host Microbe
Volume10
Issue3
Pages260-72
Date Published2011 Sep 15
ISSN1934-6069
KeywordsAdaptation, Physiological, Animals, Bacteria, Bacterial Physiological Phenomena, Clostridium, Genome, Bacterial, Humans, Intestines, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Th17 Cells
Abstract

Perturbations of the composition of the symbiotic intestinal microbiota can have profound consequences for host metabolism and immunity. In mice, segmented filamentous bacteria (SFB) direct the accumulation of potentially proinflammatory Th17 cells in the intestinal lamina propria. We present the genome sequence of SFB isolated from monocolonized mice, which classifies SFB phylogenetically as a unique member of Clostridiales with a highly reduced genome. Annotation analysis demonstrates that SFB depend on their environment for amino acids and essential nutrients and may utilize host and dietary glycans for carbon, nitrogen, and energy. Comparative analyses reveal that SFB are functionally related to members of the genus Clostridium and several pathogenic or commensal "minimal" genera, including Finegoldia, Mycoplasma, Borrelia, and Phytoplasma. However, SFB are functionally distinct from all 1200 examined genomes, indicating a gene complement representing biology relatively unique to their role as a gut commensal closely tied to host metabolism and immunity.

URLhttp://linkinghub.elsevier.com/retrieve/pii/S1931-3128(11)00255-1
DOI10.1016/j.chom.2011.08.005
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/21925113?dopt=Abstract

Alternate JournalCell Host Microbe
PubMed ID21925113
PubMed Central IDPMC3209701
Grant ListRC2 AR058986 / AR / NIAMS NIH HHS / United States
U54 HG004973 / HG / NHGRI NIH HHS / United States
U54HG004969 / HG / NHGRI NIH HHS / United States
R00 DK085329 / DK / NIDDK NIH HHS / United States
4R00DK85329-02 / DK / NIDDK NIH HHS / United States
P30 DK043351 / DK / NIDDK NIH HHS / United States
1U54HG004973-01 / HG / NHGRI NIH HHS / United States
U54 HG003273 / HG / NHGRI NIH HHS / United States
1R01HG005969 / HG / NHGRI NIH HHS / United States
/ / Howard Hughes Medical Institute / United States
R01 HG005969 / HG / NHGRI NIH HHS / United States
5RC2AR058986 / AR / NIAMS NIH HHS / United States
U54 HG004969 / HG / NHGRI NIH HHS / United States
R00 DK085329-02 / DK / NIDDK NIH HHS / United States