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Nat Rev Genet DOI:10.1038/nrg3129

Experimental and analytical tools for studying the human microbiome.

Publication TypeJournal Article
Year of Publication2011
AuthorsKuczynski, J, Lauber, CL, Walters, WA, Parfrey, LWegener, Clemente, JC, Gevers, D, Knight, R
JournalNat Rev Genet
Date Published2011 Dec 16
KeywordsAlgorithms, Computational Biology, Databases, Nucleic Acid, DNA, DNA Barcoding, Taxonomic, Genomics, Humans, Metagenome, Mouth, Nucleic Acid Amplification Techniques, RNA, Ribosomal, 16S, Skin, Software

The human microbiome substantially affects many aspects of human physiology, including metabolism, drug interactions and numerous diseases. This realization, coupled with ever-improving nucleotide sequencing technology, has precipitated the collection of diverse data sets that profile the microbiome. In the past 2 years, studies have begun to include sufficient numbers of subjects to provide the power to associate these microbiome features with clinical states using advanced algorithms, increasing the use of microbiome studies both individually and collectively. Here we discuss tools and strategies for microbiome studies, from primer selection to bioinformatics analysis.


Alternate JournalNat. Rev. Genet.
PubMed ID22179717
PubMed Central IDPMC5119550
Grant ListU54HG004969 / HG / NHGRI NIH HHS / United States
P01 DK078669 / DK / NIDDK NIH HHS / United States
R01 MD011389 / MD / NIMHD NIH HHS / United States
T32 GM008759 / GM / NIGMS NIH HHS / United States
R01 HG004872 / HG / NHGRI NIH HHS / United States
/ / Howard Hughes Medical Institute / United States
U54 HG004969 / HG / NHGRI NIH HHS / United States