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Mol Cell Proteomics DOI:10.1074/mcp.R800015-MCP200

A human proteome detection and quantitation project.

Publication TypeJournal Article
Year of Publication2009
AuthorsN Anderson, L, Anderson, NG, Pearson, TW, Borchers, CH, Paulovich, AG, Patterson, SD, Gillette, M, Aebersold, R, Carr, SA
JournalMol Cell Proteomics
Date Published2009 May
KeywordsBiomarkers, Humans, Mass Spectrometry, Pilot Projects, Proteome, Proteomics

The lack of sensitive, specific, multiplexable assays for most human proteins is the major technical barrier impeding development of candidate biomarkers into clinically useful tests. Recent progress in mass spectrometry-based assays for proteotypic peptides, particularly those with specific affinity peptide enrichment, offers a systematic and economical path to comprehensive quantitative coverage of the human proteome. A complete suite of assays, e.g. two peptides from the protein product of each of the approximately 20,500 human genes (here termed the human Proteome Detection and Quantitation project), would enable rapid and systematic verification of candidate biomarkers and lay a quantitative foundation for subsequent efforts to define the larger universe of splice variants, post-translational modifications, protein-protein interactions, and tissue localization.


Alternate JournalMol. Cell Proteomics
PubMed ID19131327
PubMed Central IDPMC2689772
Grant ListU24 CA126476 / CA / NCI NIH HHS / United States
1U24 CA126476-02 / CA / NCI NIH HHS / United States