A mitochondrial protein compendium elucidates complex I disease biology.

Cell
Authors
Keywords
Abstract

Mitochondria are complex organelles whose dysfunction underlies a broad spectrum of human diseases. Identifying all of the proteins resident in this organelle and understanding how they integrate into pathways represent major challenges in cell biology. Toward this goal, we performed mass spectrometry, GFP tagging, and machine learning to create a mitochondrial compendium of 1098 genes and their protein expression across 14 mouse tissues. We link poorly characterized proteins in this inventory to known mitochondrial pathways by virtue of shared evolutionary history. Using this approach, we predict 19 proteins to be important for the function of complex I (CI) of the electron transport chain. We validate a subset of these predictions using RNAi, including C8orf38, which we further show harbors an inherited mutation in a lethal, infantile CI deficiency. Our results have important implications for understanding CI function and pathogenesis and, more generally, illustrate how our compendium can serve as a foundation for systematic investigations of mitochondria.

Year of Publication
2008
Journal
Cell
Volume
134
Issue
1
Pages
112-23
Date Published
2008 Jul 11
ISSN
1097-4172
URL
DOI
10.1016/j.cell.2008.06.016
PubMed ID
18614015
PubMed Central ID
PMC2778844
Links
Grant list
R01 GM077465-04 / GM / NIGMS NIH HHS / United States
P30 DK043351 / DK / NIDDK NIH HHS / United States
DK43351 / DK / NIDDK NIH HHS / United States
Howard Hughes Medical Institute / United States
P30 DK057521 / DK / NIDDK NIH HHS / United States
DK57521 / DK / NIDDK NIH HHS / United States
GM077465 / GM / NIGMS NIH HHS / United States
R01 GM077465 / GM / NIGMS NIH HHS / United States