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Methods Mol Biol DOI:10.1007/978-1-60761-845-4_4

Detecting protein-small molecule interactions using fluorous small-molecule microarrays.

Publication TypeJournal Article
Year of Publication2010
AuthorsVegas, AJ, Koehler, AN
JournalMethods Mol Biol
Volume669
Pages43-55
Date Published2010
ISSN1940-6029
KeywordsBiocatalysis, Drug Evaluation, Preclinical, HEK293 Cells, Histone Deacetylase Inhibitors, Histone Deacetylases, Histones, Humans, Hydrolysis, Microarray Analysis, Nuclear Receptor Co-Repressor 2, Printing, Protein Binding, Proteins, Small Molecule Libraries
Abstract

General binding assays involving microarrays of small molecules can be used to identify small molecule ligands for nearly any protein, even in the absence of knowledge about protein structure or function. Several suitable methods for manufacturing small molecule microarrays (SMMs) exist and different immobilization methods may be more or less preferable for any given application. Here, we describe a protocol for noncovalent and homogenous capture of small molecules using fluorous interactions between small molecules containing fluorocarbon tags and fluorocarbon-coated glass surfaces. These arrays are especially useful for applications that require display of compounds in a specific orientation such as screening biased libraries.

URLhttp://dx.doi.org/10.1007/978-1-60761-845-4_4
DOI10.1007/978-1-60761-845-4_4
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/20857356?dopt=Abstract

Alternate JournalMethods Mol. Biol.
PubMed ID20857356
Grant ListN01-CO-12400 / CO / NCI NIH HHS / United States