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Science DOI:10.1126/science.1093139

Genetic dissection of complex traits with chromosome substitution strains of mice.

Publication TypeJournal Article
Year of Publication2004
AuthorsSinger, JB, Hill, AE, Burrage, LC, Olszens, KR, Song, J, Justice, M, O'Brien, WE, Conti, DV, Witte, JS, Lander, ES, Nadeau, JH
Date Published2004 Apr 16
KeywordsAmino Acids, Animals, Anxiety, Chromosome Mapping, Chromosomes, Mammalian, Crosses, Genetic, Diet, Female, Genetic Predisposition to Disease, Genetic Variation, Male, Mice, Mice, Inbred A, Mice, Inbred C57BL, Microsatellite Repeats, Obesity, Phenotype, Quantitative Trait Loci, Quantitative Trait, Heritable, Sterols, Weight Gain

Chromosome substitution strains (CSSs) have been proposed as a simple and powerful way to identify quantitative trait loci (QTLs) affecting developmental, physiological, and behavioral processes. Here, we report the construction of a complete CSS panel for a vertebrate species. The CSS panel consists of 22 mouse strains, each of which carries a single chromosome substituted from a donor strain (A/J) onto a common host background (C57BL/6J). A survey of 53 traits revealed evidence for 150 QTLs affecting serum levels of sterols and amino acids, diet-induced obesity, and anxiety. These results demonstrate that CSSs greatly facilitate the detection and identification of genes that control the wide diversity of naturally occurring phenotypic variation in the A/J and C57BL/6J inbred strains.


Alternate JournalScience
PubMed ID15031436
Grant ListGM07250 / GM / NIGMS NIH HHS / United States
HD07518 / HD / NICHD NIH HHS / United States
RR12305 / RR / NCRR NIH HHS / United States