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Curr Opin Chem Biol DOI:10.1016/j.cbpa.2004.04.002

Complex phenotypic assays in high-throughput screening.

Publication TypeJournal Article
Year of Publication2004
AuthorsClemons, PA
JournalCurr Opin Chem Biol
Volume8
Issue3
Pages334-8
Date Published2004 Jun
ISSN1367-5931
KeywordsCombinatorial Chemistry Techniques, Drug Design, Drug Evaluation, Preclinical, Genetic Engineering, Medical Informatics, Pattern Recognition, Automated, Phenotype, Spectrometry, Fluorescence
Abstract

High-throughput screening (HTS), systematically testing thousands of small molecules to find candidates for lead optimization, primarily involves exposure of purified proteins to arrayed collections of small molecules. More complex phenotypic assays, such as cell-based or whole-organism assays, traditionally have flanked HTS, preceding it to validate new therapeutic targets, and following it to characterize new lead compounds in cellular contexts. Recently, however, cell- and organism-based phenotypic assays have increasingly been adopted as a primary screening platform for annotating small molecules.

URLhttp://linkinghub.elsevier.com/retrieve/pii/S1367593104000481
DOI10.1016/j.cbpa.2004.04.002
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/15183333?dopt=Abstract

Alternate JournalCurr Opin Chem Biol
PubMed ID15183333