Scientific Publications

Enhancing linkage analysis of complex disorders: an evaluation of high-density genotyping.

Publication TypeJournal Article
AuthorsSawcer, SJ, Maranian M., Singlehurst S., Yeo T., Compston A., Daly M. J., De Jager PL, Gabriel S., Hafler DA, Ivinson AJ, Lander E. S., Rioux JD, Walsh E., Gregory SG, Schmidt S., Pericak-Vance MA, Barcellos L., Hauser SL, Oksenberg JR, Kenealy SJ, and Haines JL
AbstractTo explore the potential value of recently developed high-density linkage mapping methods in the analysis of complex disease we have regenotyped five nuclear families first studied in the 1996 UK multiple sclerosis linkage genome screen, using Applied Biosystems high-density microsatellite linkage mapping set, the Illumina BeadArray linkage mapping panel (version 3) and the Affymetrix GeneChip Human Mapping 10K array. We found that genotyping success, information extraction and genotyping accuracy were improved with all systems. These improvements were particularly marked with the SNP-based methods (Illumina and Affymetrix), with little difference between these. The extent of additional information extracted is considerable, indicating that reanalysis of existing multiplex families using these newer systems would substantially increase power.
Year of Publication2004
JournalHuman molecular genetics
Volume13
Issue17
Pages1943-9
Date Published (YYYY/MM/DD)2004/09/01
ISSN Number0964-6906
DOI10.1093/hmg/ddh202
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/15238506?dopt=Abstract