Enhancing linkage analysis of complex disorders: an evaluation of high-density genotyping.

Hum Mol Genet
Authors
Keywords
Abstract

To explore the potential value of recently developed high-density linkage mapping methods in the analysis of complex disease we have regenotyped five nuclear families first studied in the 1996 UK multiple sclerosis linkage genome screen, using Applied Biosystems high-density microsatellite linkage mapping set, the Illumina BeadArray linkage mapping panel (version 3) and the Affymetrix GeneChip Human Mapping 10K array. We found that genotyping success, information extraction and genotyping accuracy were improved with all systems. These improvements were particularly marked with the SNP-based methods (Illumina and Affymetrix), with little difference between these. The extent of additional information extracted is considerable, indicating that reanalysis of existing multiplex families using these newer systems would substantially increase power.

Year of Publication
2004
Journal
Hum Mol Genet
Volume
13
Issue
17
Pages
1943-9
Date Published
2004 Sep 01
ISSN
0964-6906
URL
DOI
10.1093/hmg/ddh202
PubMed ID
15238506
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