Transcriptional regulatory code of a eukaryotic genome.

Nature
Authors
Keywords
Abstract

DNA-binding transcriptional regulators interpret the genome's regulatory code by binding to specific sequences to induce or repress gene expression. Comparative genomics has recently been used to identify potential cis-regulatory sequences within the yeast genome on the basis of phylogenetic conservation, but this information alone does not reveal if or when transcriptional regulators occupy these binding sites. We have constructed an initial map of yeast's transcriptional regulatory code by identifying the sequence elements that are bound by regulators under various conditions and that are conserved among Saccharomyces species. The organization of regulatory elements in promoters and the environment-dependent use of these elements by regulators are discussed. We find that environment-specific use of regulatory elements predicts mechanistic models for the function of a large population of yeast's transcriptional regulators.

Year of Publication
2004
Journal
Nature
Volume
431
Issue
7004
Pages
99-104
Date Published
2004 Sep 02
ISSN
1476-4687
URL
DOI
10.1038/nature02800
PubMed ID
15343339
PubMed Central ID
PMC3006441
Links
Grant list
R01 HG002668 / HG / NHGRI NIH HHS / United States
R01 HG002668-01 / HG / NHGRI NIH HHS / United States
R01 HG004037 / HG / NHGRI NIH HHS / United States