Scientific Publications

Transcriptional regulatory code of a eukaryotic genome.

Publication TypeJournal Article
AuthorsHarbison, CT, Gordon DB, Lee TI, Rinaldi NJ, Macisaac KD, Danford TW, Hannett NM, Tagne JB, Reynolds DB, Yoo J., Jennings EG, Zeitlinger J., Pokholok DK, Kellis M., Rolfe PA, Takusagawa KT, Lander E. S., Gifford DK, Fraenkel E., and Young RA
AbstractDNA-binding transcriptional regulators interpret the genome's regulatory code by binding to specific sequences to induce or repress gene expression. Comparative genomics has recently been used to identify potential cis-regulatory sequences within the yeast genome on the basis of phylogenetic conservation, but this information alone does not reveal if or when transcriptional regulators occupy these binding sites. We have constructed an initial map of yeast's transcriptional regulatory code by identifying the sequence elements that are bound by regulators under various conditions and that are conserved among Saccharomyces species. The organization of regulatory elements in promoters and the environment-dependent use of these elements by regulators are discussed. We find that environment-specific use of regulatory elements predicts mechanistic models for the function of a large population of yeast's transcriptional regulators.
Year of Publication2004
JournalNature
Volume431
Issue7004
Pages99-104
Date Published (YYYY/MM/DD)2004/09/02
ISSN Number0028-0836
DOI10.1038/nature02800
PubMedhttp://www.ncbi.nlm.nih.gov/pubmed/15343339?dopt=Abstract