|Publication Type||Journal Article|
|Year of Publication||2005|
|Authors||Chang, C-Y, LaBaer, J|
|Date Published||2005 May 01|
|Keywords||Cloning, Molecular, Database Management Systems, Databases, Nucleic Acid, DNA Mutational Analysis, DNA, Complementary, Gene Frequency, Information Storage and Retrieval, Polymorphism, Genetic, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Software, User-Computer Interface|
SUMMARY: DNA polymorphism detector (DPD) is a new web application developed to help automate the process of cDNA clone validation. DPD identifies and highlights discrepancies between any cDNA clone sequence and its expected reference sequence. To determine if these differences correspond to natural genetic polymorphisms (versus artifacts introduced during clone production or evaluation), DPD uses the discrepancies, along with flanking sequences, to search GenBank for identical matching strings. If matching DNA sequences are found, DPD verifies that they are from the same gene. The application then reports the discrepancy as a polymorphism along with the corresponding GenBank reference information.
AVAILABILITY: DPD is currently hosted by the Harvard Institute of Proteomics at http://www.hip.harvard.edu