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Current opinion in chemical biology DOI:10.1016/j.cbpa.2004.10.009

Using genome-wide transcriptional profiling to elucidate small-molecule mechanism.

Publication TypeJournal Article
Year of Publication2005
AuthorsButcher, RA, Schreiber, SL
JournalCurrent opinion in chemical biology
Volume9
Issue1
Pages25-30
Date Published2005/02/01
ISSN1367-5931
Abstract

Transcriptional profiling with DNA microarrays can be used to measure the genome-wide transcriptional response to small molecules. Recent progress in the analysis of gene-expression data has relied on the generation of databases of profiles documenting the transcriptional effects of various compound treatments and genetic perturbations. A positive correlation between the transcriptional response induced by a novel small molecule and a database profile can provide insight into the molecule's mechanism. Transcriptional profiling can also be used to assess a small molecule's specificity for its target and to facilitate analysis of pathways downstream of the target.

URLhttp://linkinghub.elsevier.com/retrieve/pii/S1367-5931(04)00142-5
DOI10.1016/j.cbpa.2004.10.009
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/15701449?dopt=Abstract