Mass spectrometry-based proteomics turns quantitative.

Nat Chem Biol
Authors
Keywords
Abstract

The field of proteomics is built on technologies to analyze large numbers of proteins--ideally the entire proteome--in the same experiment. Mass spectrometry (MS) has been successfully used to characterize proteins in complex mixtures, but results so far have largely been qualitative. Two recently developed methodologies offer the opportunity to obtain quantitative proteomic information. Comparing the signals from the same peptide under different conditions yields a rough estimate of relative protein abundance between two proteomes. Alternatively, and more accurately, peptides are labeled with stable isotopes, introducing a predictable mass difference between peptides from two experimental conditions. Stable isotope labels can be incorporated 'post-harvest', by chemical approaches or in live cells through metabolic incorporation. This isotopic handle facilitates direct quantification from the mass spectra. Using these quantitative approaches, precise functional information as well as temporal changes in the proteome can be captured by MS.

Year of Publication
2005
Journal
Nat Chem Biol
Volume
1
Issue
5
Pages
252-62
Date Published
2005 Oct
ISSN
1552-4450
URL
DOI
10.1038/nchembio736
PubMed ID
16408053
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