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Genome Biol DOI:10.1186/gb-2006-7-7-r61

A method for high-throughput gene expression signature analysis.

Publication TypeJournal Article
Year of Publication2006
AuthorsPeck, D, Crawford, ED, Ross, KN, Stegmaier, K, Golub, TR, Lamb, J
JournalGenome Biol
Volume7
Issue7
PagesR61
Date Published2006
ISSN1474-760X
KeywordsBase Sequence, DNA Primers, Gene Expression Profiling, HL-60 Cells, Humans, Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis, Reproducibility of Results, RNA, Messenger, Transcription, Genetic
Abstract

Genome-wide transcriptional profiling has shown that different biologic states (for instance, disease and response to pharmacologic manipulation) can be recognized by the expression pattern of relatively small numbers of genes. However, the lack of a practical and cost-effective technology for detection of these gene expression 'signatures' in large numbers of samples has severely limited their exploitation in important medical and pharmaceutical discovery applications. Here, we describe a solution based on the combination of ligation-mediated amplification with an optically addressed microsphere and flow cytometric detection system.

URLhttp://genomebiology.com/content/7/7/R61
DOI10.1186/gb-2006-7-7-r61
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/16859521?dopt=Abstract

Alternate JournalGenome Biol.
PubMed ID16859521
PubMed Central IDPMC1779561